FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4490727
Sequences flagged as poor quality0
Sequence length250
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGAGGTCTCAGAGAGGAGCCTTAGC2754086.132815466181756Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAACAACCACATCCCTCCTC1757903.9145109466685457Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGCTTTCTGAGAGTCACGGACC1546923.444698375118327Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCAGCA1307182.910842721011542Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGACGAGCCCAGCA1240932.7633164964158365Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTAGAAG1044032.3248574228627126Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACCATGGACTGGACCTGGAGGGTC953702.123709590896975Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAAATACTTTCTGAGAGTCCTGGA939212.09144310041559Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACATAACAGCCAGATTCCTC925342.0605572327153263Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTTTCTGAGAGTCATGGATC906512.0186263827660866Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC903402.0117010007511036Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAACAACCACACCCCTCCTAA850771.8945039411213374Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTCTGAGAGTCTTGGACCTCC837011.8638630226241766Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGCCCCAGCCCTGGGATTCC821271.8288130184711742Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAACAACCACACCCCTCCTA810021.80376139542662Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTCTCTGAGAGTCATGGATC797431.775725845726093Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGAGGAGCCCAGCACTGGGATTCC782721.742969456838503Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGGGAGCGGAGCCCCAGCCGTG732941.6321188083800242Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCATCACATAACAACCAGATTCC724411.613124111084909Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCC712421.5864246479467579Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT702721.5648245818550093Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGACGAGCCCAGCACTGGAAGT663831.4782239045036583Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACACAACAGCCACATCCCTC658181.465642422707949Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGTGGGGAGAGGAGCCCAGCACTGGG652321.452593310615408Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCCAGCCCTGAGA627291.3968562328549476Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGACGAGCCCAGCACTGGAA625361.3925584877459707Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCCTCGTTGCTCTTTTAAGAGGTGT621841.3847201132466969Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGTCCAGCAC523511.1657577937826102Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGTCTCAGAGAGGAGCCTTAGCC523371.1654460402513893Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCCTCGGGATTCCCAGCTGTCA511131.1381898743789147Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCCAGC496021.1045427611164071Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAGGAGCCCAGCCCTGGGAT494881.1020041966478924Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAAAGGAGCCCAGCAC488201.0871290995867706Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGCTTTCTGAGAGTCACGGAC456781.0171626999370036Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGAGAGAGGAGCCTTAGCCCTG403990.8996093505572705Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCCAGCCCTGAGA402120.8954452141045314Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAAATACTTTCTGAGAGTCCTG331270.7376756591972747Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGACGAGCCCAGCACTGGAAG326100.7261630466514665Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGACGAGCCCAGCAC299770.6675311146725241Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCCAGCC235690.5248370698107456Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAAGAGCCCCAGC218500.4865581897986674Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACGTGGTCGGGGAAGAAGCCTGGG78880.17565084673372486Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCCAGCCTGGGGAGTCCCTGAG78330.17442610071821332Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGCCAGGGAACCCTGGTCACCG47090.10486052703715901Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)