FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4624845
Sequences flagged as poor quality0
Sequence length250
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGGAGCCCCAGCCCTGGGATTCCCA3726748.058086270999352Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCCCCAGCCCTGGGATTCCCAG2163194.677324321139411Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGAGCCCCAGCCCTGGGATTCC1759673.8048194047584296Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTGGGAGAGGAGCCCCAGCCCTG1579903.4161144859989903Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGCCCCAGCCCTGGGATTCCC1504773.253665798529464Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCC1232632.6652352673440944Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACAGGGCAGGGGCACAGGCTCGGG1103542.3861123994425757Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCACAGGGCAGGGGCACAGGCTC1099142.3765985670871133Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCCAGCC935672.0231380727354105Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCCCCAGCCCTGGGATTCCCAGC831911.79878460791659Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGCTCAGGAGAAGGACAGAGCC819471.771886409166145Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCCAGCCCTGGGA806281.7433665344460194Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCGGCGCTCCTGGACCTGAGCT783501.6941108296602372Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGCATCACATCACAACCACAT780731.6881214397455484Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCGCCACTAGAA727871.573825717402421Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGACAGAAGACTCATCATGGAGTTCTG693731.5000070272625354Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC671651.4522648867151222Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAACTCACCATGGAGTTTGGG587751.270853401573458Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTGGGAGAGGAGCCCCAGCCT568601.2294466084809328Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATGCTTTCTGAGAGTCATGGATC567461.2269816610070177Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTCTTTGCAGCGTGGAGTTCAC548801.1866343628813507Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCCCCAGCCCTGGGATTCCCAGCT511121.10516136216457Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGTCACGCACCGACAGAGGACTCACCA506461.0950853488062844Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCGGCTCCCAGATGGGTCCTGTC470671.0176989715331Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTCTGAGAGTCATGGATCTC460340.995363087844025Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAAGGCATTTCCACTTGGTGATCA449050.9709514589137581Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCTTAGC444130.9603132645526499Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCGCCAC411380.8895000805432398Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCCAGCCCTGAG408760.8838350258224871Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCTACTCCCTCAGCAGCGTGGA373100.8067297390507142Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCC354840.7672473347755439Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACTGGACCTGGAGGTTCCTCTT350080.7569550979546341Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATTTTCTGGGAGAGGAGCCCCAGC323140.6987044971236873Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGTCTGAGAGAGGAGCCCAGCCCTGG321650.6954827675305875Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTGAGAGAGGAGCCCAGCCCT314040.6790281620248895Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTGGGAGAGGAGCCCAGCACT311070.6726063251849521Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGAGCCCAGCCCTGGGATTTT310510.6713954737942569Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGAGGAGCCTTAGCCCTGGA247260.5346341336844802Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACTCCGCAGCTCTGGGAGAGGA220880.47759438424422873Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTGGGAGAGGAGCCCGCCACT203230.4394309430910658Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGAGGAGCCCAGCAC200090.4326415263646673Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGAGCCCCAGCCCTGGGATTC190250.41136513764245075Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTTCTGGGAGAGGAGCCCCAGCCC182190.393937526554944Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTCTGAGAGTCATGGACCTC150390.3251784654404634Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTGTTTCCGTTCAGTGATCAG123280.26656028472305554Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCCCCAGCCCTGGGATTCCCAGCTG122650.26519807690852343Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGGCTGGGTACAGCCTGGCG110690.23933775077867475Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCTTAGTTCAGCCTGGGGGGTCCCT108980.23564032956780173Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTTCGGGGGACCCACCAGAAGCC60960.13180982281568357Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGGCAATTTATGAACCCGAAAG52790.11414436591929028Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)