FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001880405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970879
Sequences flagged as poor quality0
Sequence length250
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGGTATCAACGCAGAGTACGGGGAGGTCTCAGAGAGGAGCCTTAGC43118214.513617013685176Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACACAACAGCCACATCCCTC1962116.604476318288291Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGGGAGAGGAGCCCAGCA1342304.51819141742225Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAACAACCACATCCCTCCTC1273304.285936923045335Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGGTCTCAGAGAGGAGCCTTAGCC1039953.500479151119921Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGAGAGGAGCCCAGCACTAGAAG644612.1697618785551347Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCCAAAAACCACACCCCTCCTT633162.131221096517226Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCAC577871.945114560370853Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCTTAGC542321.825453005659268Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAACAACCACACCCCTCCTAA492841.6589029711408643Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGGAGAGGAGCCCAGCACTAGAA416241.401066822310838Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAACCACATCCCTCCTCAGAAGCC405251.3640744035687755Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCTCAGAGAGGAGCCTTAGCCC359711.2107864372800103Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGCTCGGGAGGAGAAGGACAGA349491.1763858440549078Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTG321751.0830128053010573Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTCAGAGAGGTGCCTTAGC286000.9626780491564955Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGATCACACAACAGCCACATCCCTCC283840.9554074736803485Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGGAAATGCTTTCTGAGAGTCA278530.9375339756348207Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCCCACATGTACCTCGCCACAAC274460.9238343264737473Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCATGGACTGGACCTGGAGGATC263540.8870775282332266Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTTTCTGAGAGTCATGGATCTC261210.8792347315390495Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCTGAACACAGAGGACTCACCATGGA255510.8600484906992173Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGGACTGAACAGAGAGAACTCACC240070.8080773400734261Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGCGGTGTTTCCATTCGGTGATCAGC229920.7739123673498651Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGTGCTTTCTGAGAGTCCTGGAC217920.7335202813712709Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCATCACCCAGCAACCACATC215980.7269902274713982Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCCTTAGCCCTGGACTCCAAG215030.7237925206647595Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTCTGGGAGAGGAGCCCAGCACTA214010.7203591933565789Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGATCACCCAAAAACCACACCCCTC207500.6984464867131915Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGACCCAAAAACCACACCCCTCCT205040.6901661090875798Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGGTCTCAGAGAGGAGCCTTAGCC195660.6585929618809786Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGAGGAGCCTTAGCCCTGGACT190790.6422005069879992Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGTCTCAGAGAGGAGCCTTAG190250.6403828631189624Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGCTCTGAGAGAGGAGCCCAGCC177310.5968267304053784Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCCCCTAGATCACAGCTCCTCACC175610.5911045182250775Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAGGAGCCTTAGCCCTGGAT170190.5728607593914125Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGGGAGAGGAGCCCAGCAC165240.5561990239252423Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCTCTGAGAGAGGAGCCTTAGCC153040.5151337365136716Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCTCTGAGAGAGGAGCCCAGC148250.4990105621938827Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGAGAGGAGCCTTAGCCCTGGAC142410.47935308035096685Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCC142270.4788818393478832Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCTCTGGGAGAGGAGCCCAGCACT137250.4619844833801713Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGACCACCCAAAAACCACACCCCTCC122230.4114270557636309Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCTCTGGGAGTGGAGCCCCAG120790.4065800054461996Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGAGGTCTGGGAGAGGAGCCCAGCA104360.35127650772717434Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGACCATCACACAACAGCCACATCC102670.3455879556185223Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTGGTGTTTCCATTCGGTGATCA96180.32374256911843263Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGATCACCCAACAACCACATCCCT92890.31266840554596803Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGCACGTGGTCGGGGAAGAAGCCTGGG91650.3084945566615133Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGCATCACACAACAGCCACATCCCT86540.2912942600489619Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGCCTTAGCCCTGGATTCCAAGGC85310.28715407123615605Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGTGGGAGAGGAGCCCAGCACTAGA75560.25433550137854827Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGGAGCCCCAGCCCTGGGATTC74820.25184465607653495Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGAGCTCTGGGAGAAGAGCCCCAG49490.1665836945900523Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACGGGGGTCACCACGCTGCTGAGGTGAAG44530.14988829905223336Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAAGTACGGGGGCTTTCTGAGAGTCATGGATCTCA33250.11191973823235479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)