FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523380
Sequences flagged as poor quality0
Sequence length251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA847325.562105318436634No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT718684.7176672924680645No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA623614.093594506951647No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA558073.6633669865693395No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT476883.130407383581247No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA358392.3525975134240964No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT288611.8945371476584962No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA287981.8904016069529601No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA267551.756291929787709No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA246171.615946119812522No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA237871.561462012104662No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA197081.2937021622969973No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT193231.2684294135409417No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGT175581.152568630282661No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA173291.1375362680355525No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA162551.0670351455316467No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT154631.0150454909477609No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGT120490.790938570809647No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCACAC117090.768619779700403No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT106090.6964119261116728No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT103730.680920059341727No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT81550.5353227691055417No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA80620.5292179233021308No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA77100.5061114101537371No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCACAC75380.49482072759259016No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT74760.49075083039031625No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA74260.4874686552271921No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA74180.48694350720109236No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA70310.4615394714385117No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGAGCATTGT69060.4533340335307015No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGT67260.4415182029434547No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT61320.40252596200554036No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA58850.3863120166997072No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT57380.3766624217201224No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA55640.3652404521524505No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT53230.34942036786619224No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGAGCATTGT51430.3376045372789455No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA51300.33675117173653324No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT50100.3288739513450354No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT46560.3056361511901167No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA46500.30524229017054183No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGAGT45780.3005159579356431No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGT45210.2967742782496816No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC42900.2816106289960483No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA41780.27425855663065024No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCGTGGT41610.2731426170751881No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT40300.2645433181478029No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT40060.26296787406950334No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA39450.2589636203704919No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT37350.24517848468537068No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGCGACGGTGACCGTGGT36050.236644829261248No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA35360.23211542753613676No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT34290.22509157268705118No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT33960.22292533707938927No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGAGT32140.2109782194856175No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGCGACCAGGGT30570.2006721894734078No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT30550.20054090246688286No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT30450.19988446743425806No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCGTGGT30010.19699615329070883No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA29640.19456734366999698No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA29000.19036615946119811No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA28740.1886594283763736No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC28340.1860336882458743No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT27390.1797975554359385No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA27040.17750003282175164No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT26730.1754650842206147No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA25270.16588113274429228No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGCGACGGTGACCGTGGT25160.16515905420840502No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT24010.15761005133321956No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCACGGT23840.15649411177775735No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT23780.15610025075818246No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT22990.1509144140004464No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT22770.14947025692867177No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA22600.14835431737320956No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA22240.14599115125576023No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA20570.1350286862109257No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA20260.13299373760978878No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT19850.130302353976027No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT19400.1273483963292153No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGATCAGCGT18910.12413186466935368No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACACTGACCAGGGT18840.1236723601465163No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA18740.12301592511389148No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGCGACCAGGGT18670.1225564205910541No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT18500.12144048103559192No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT18480.12130919402906695No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT18320.12025889797686723No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18250.11979939345402985No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTG18140.11907731491814255No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA17630.11572949625175596No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT17560.1152699917289186No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA17150.11257860809515682No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT17120.11238167758536938No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA16810.11034672898423244No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT16640.10923078942877024No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTG16460.10804920637004557No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA15900.10437317018734656No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT15760.10345416114167182No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA15650.10273208260578451No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT15570.10220693457968465No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCACGGT15380.10095970801769749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)