FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424140
Sequences flagged as poor quality0
Sequence length251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1032777.251885348350584No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT787865.532180824918898No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA665304.6715912761385825No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT333962.3449941719213No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA318222.234471330065864No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT256161.798699566053899No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC254111.78430491384274No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA238581.6752566461162526No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA209201.4689567036948614No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC196271.3781650680410633No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA137870.9680930245621919No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA134330.9432359178170686No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA125820.8834805566868427No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT111200.7808221101857964No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGC103690.7280885306219894No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGATCAGGGA101860.7152386703554425No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT86130.6047860463156712No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT86070.6043647394216861No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA86020.6040136503433652No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT83270.5847037510357127No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGC81350.5712219304281883No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGATCAGGGA77560.5446093782914602No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGT74200.5210161922282921No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA71570.5025489067086101No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT66360.4659654247475669No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT65840.4623140983330291No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT65790.46196300925470807No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA62390.4380889519288834No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT60850.4272754083165981No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA57520.40389287570042276No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGT57150.4012948165208477No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT56980.4001011136545564No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT56080.3937815102447793No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT55750.39146432232786105No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA51250.35986630527897534No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT50680.35586388978611655No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT49770.3494740685606752No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT49310.34624404904012246No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGATCAGGGA48400.3398542278146811No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT48280.3390116140267109No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT46780.3284789416770823No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGGGGT46070.3234934767649248No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA45250.3177356158804612No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT45050.3163312595671774No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT45020.3161206061201848No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT44970.31576951704186385No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA43760.30727316134649685No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT41700.2928082913196736No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGC41190.28922718272079995No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA38760.27216425351440166No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT38380.2694959765191624No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTTGGT38130.2677405311275577No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGTAGAGGGTGACCAGGGT37850.26577443228896036No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT37740.26500203631665425No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTAACCGCGGT37350.2622635415057508No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT37100.26050809611414605No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGATCAGGGA36370.2553821955706602No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT36250.25453958178268987No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGTAGGGT35740.2509584731838162No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGGGGT35170.24695605769095735No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT34870.24484952322103162No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT34750.24400690943306136No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT34670.24344516690774787No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGATGACGGTGGT34330.2410577611751654No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT32890.23094639571952197No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA32830.23052508882553682No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGC31250.21943067395059473No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT30330.21297063490948923No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTTGGT29290.20566798208041343No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTAACCGCGGT29160.20475515047677895No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT28340.19899728959231539No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACAATTAGGAGGGT28090.1972418442007106No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGTAGAGGGTGACCAGGGT28070.19710140856938224No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA27560.1935202999705085No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGATGACGGTGGT27310.19176485457890374No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGTAGGGT26650.1871304787450672No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT26630.18699004311373882No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGTGCT26540.18635808277276109No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA26110.18333871669920093No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT25960.18228544946423808No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCACAGT25710.1805300040726333No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGATGAGACAATAAGGAGGGT25270.17744042018340894No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTAACCATTGT23410.16437990646986955No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGGAGT22520.1581305208757566No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC22280.15644529329981602No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT22070.15497071917086805No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT21660.15209178872863624No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA21550.15131939275633013No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAAGAGACAATTAGGAGGGT21380.1501256898900389No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT21220.14900220483941184No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGTGCT21050.14780850197312062No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT20580.14450826463690367No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA20500.14394652211159015No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGATGGTGACCAGGGT20500.14394652211159015No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT19810.139101492830761No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGATGAGACAATAAGGAGGGT19690.13825887904279072No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT19650.13797800778013397No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT19640.13790778996446978No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCACAGT19470.13671408709817856No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTAACCATTGT18840.13229036471133457No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA18090.12702402853652028No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGAT17790.12491749406659458No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGGAGT17750.12463662280393782No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCAGGGT17430.12238965270268372No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCAT17410.12224921707135535No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA17320.12161725673037764No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT16850.11831701939416069No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGT16490.11578917803024982No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT16430.11536787113626469No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT16280.11431460390130184No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA16280.11431460390130184No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT16250.11410395045430925No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTTAGGTCCTCCAGCACTGAGA16250.11410395045430925No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGTT16170.11354220792899575No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGT16140.11333155448200317No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGATGGTGACCAGGGT15990.11227828724704031No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC15790.11087393093375653No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC15630.10975044588312946No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA15340.10771412922886796No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT15240.10701195107222605No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT15060.10574803039027061No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT14550.10216692179139691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)