FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590338
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1116817.022469437314584No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT855215.377536096100326No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA596623.7515295490644127No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA511363.2154171000127016No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT493103.1005987406450704No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT395262.4853836102765574No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT371572.336421565730052No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA328882.0679880629149276No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT317721.997814301110833No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT287871.8101183521993438No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT253341.5929946967248472No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA210831.3256930287775304No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA177181.1141027882123171No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA152730.9603618853350671No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA139470.8769833834065462No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGT117720.7402200035464159No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA115490.7261978271285727No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA109730.6899791113587174No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA105680.664512826833038No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA104300.6558354261798435No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA103560.6511823272788552No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA100600.6325699316749018No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGT92650.5825805583467163No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT91960.578241858020119No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT90380.5683068630693601No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA87020.5471792788702778No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA85800.5395079536551349No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGC81530.5126583154021347No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT72320.4547460979992932No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT70290.4419815158790144No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT70150.4411011998707193No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGC65970.414817479051623No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT65900.4143773210474755No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT64230.4038764086628126No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT63090.3967081211666954No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA53710.3377269486109242No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT52850.3323192931313972No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT52680.3312503379784675No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT52540.3303700219701724No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT51110.3213782227425868No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT49550.3115689872215843No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT47840.3008165559774086No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTAACCGCGGT45630.2869201389893218No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA45490.2860398229810267No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT43490.2734638800053825No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTA42500.26723878823243863No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA42380.2664842316539No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT41400.26032201959583434No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGGGGAGACGGTCACCACGGT41030.2579954701453402No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT40990.2577439512858273No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT40270.25321661181459537No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA39750.2499468666409279No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGAGT37470.23561029164869354No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA36630.23032839559892299No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTAACCGCGGT36380.22875640272696746No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT36180.22749880842940304No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCCAAAT35540.22347450667719693No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACAAGGGT35280.22183963409036317No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT34190.21498574516863714No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT33920.21328799286692515No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGGGGAGACGGTCACCACGGT33780.21240767685863005No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT32950.20718866052373774No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA32640.20523938936251288No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT31720.19945445559371658No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGT31180.19605895099029264No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGATCAGGGT31150.19587031184565795No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGTT31060.19530439441175398No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC30930.1944869581183371No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT30820.19379528125467668No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT30310.19058841579588742No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATG29340.1844890834527No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGCGACAAGAGT29320.18436332402294353No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGT29230.18379740658903956No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGAGT28840.18134509770878895No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACAAGGGT28650.18015038312610276No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA28290.1778867133904868No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCCAAAT28080.17656623937804417No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGT27770.17461696821681932No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA27570.1733593739192549No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGACGACCATTGT27010.16983810988607453No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT26920.16927219245217054No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGTT25750.1619152658114187No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT25740.16185238609654048No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGATCAGGGT25720.16172662666678403No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCGTTGT25180.1583311220633601No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGCAGGGAGATAACCTCTTGG25100.15782808434433435No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT24900.15657049004676993No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA24850.15625609147237882No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT24640.1549356174599362No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGT24270.15260906800944202No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT24100.15154011285651228No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC24080.15141435342675583No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23890.1502196388440696No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATG23890.1502196388440696No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23880.1501567591291914No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT23710.14908780397626165No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGCGACAAGAGT23100.1452521413686902No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGT22830.1435543890669782No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCCTTGT22540.1417308773355098No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT21800.13707777843452149No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCGTAAT21470.13500274784354016No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGCGACCACTGT21080.13255043896328958No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA21040.1322989201037767No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACTAGGGT20960.13179588238475093No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT20840.13104132580621228No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT20440.12852613721108344No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCGTTGT20240.12726854291351902No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGACGACCATTGT20200.12701702405400614No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT19900.1251306326076595No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGAGAGACGGTGACCATTGT19860.12487911374814663No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT19660.1236215194505822No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGT19610.12330712087619111No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCCTTGT19000.11947145826861963No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGCAGGGAGATAACCTCTTGG18850.11852826254544631No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT18700.117585066822273No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18680.11745930739251655No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT18640.11720778853300368No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18420.11582443480568283No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGAGGGGCTAG18310.11513275794202238No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA18180.11431532164860553No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCGTAAT18120.11393804335933619No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGCGT18100.11381228392957975No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACTAGGGT17920.11268044906177178No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGAT17700.11129709533445092No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT17670.11110845618981625No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGATGACCAGGGT17390.10934782417322607No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACAGTGACCAGCGT17120.1076500718715141No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA17020.10702127472273189No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGCGACCACTGT16890.10620383842931502No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACTAGGGT16760.10538640213589814No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCATAGT16720.10513488327638527No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT16220.10199089753247423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)