FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097176
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT664706.058280531108956No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT489754.4637323455853934No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA340933.1073410282397718No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT308932.8156831720708437No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT234622.1383989441985607No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA229022.0873588193689985No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA179091.6322814206654175No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA137381.25212363376523No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA97040.8844524488322748No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT96490.8794395794293715No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA95600.8713278453046732No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA79750.7268660634210008No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC76090.6935076961216796No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT72730.6628836212239422No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGC65630.5981720343864613No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTAACCGCGGT59030.5380176015516198No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA58740.5353744522300888No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC57130.5207004163415897No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT55610.5068466681735656No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGT53270.4855191874412127No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGC49880.45462168330331687No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT45800.41743530664177847No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAAT44800.4083209986364995No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTAACCGCGGT44080.40175869687269866No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT42450.38690237482409384No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT42340.38589980094351317No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT42130.3839857962624046No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT41970.38252750698155996No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGT40280.3671243264526384No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGATCAGGGT39370.3588303061678345No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT37840.34488541491975766No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT36090.32893537591051936No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA35480.3233756480272992No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT34600.31535505698265365No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT34510.3145347692621785No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAAT34480.31426134002202016No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT34300.31262076458106997No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT34200.31170933378054205No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT32810.2990404456532042No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT32710.2981290148526763No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA32360.2949390070508287No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT31950.2912021407686643No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT31650.2884678483670806No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGGGGT31480.2869184160061831No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGATCAGGGT30480.2778041080009041No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT29870.2722443801176839No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT29780.2714240923972088No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT29030.2645883613932496No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA28860.26303892903235215No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT28760.26212749823182424No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT26810.2443545976215302No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT26410.24070887441941857No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT25690.23414657265561767No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAACGACGGCGGT25340.23095656485377006No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT25240.23004513405324215No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT24870.22667284009128894No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT24310.22156882760833269No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT23600.21509766892458454No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT23250.21190766112273693No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGGGGT23130.21081394416210342No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA23080.2103582287618395No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACAATGACCAGGGT22820.20798850868046695No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT21570.19659562367386815No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT21550.1964133375137626No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT21250.1936790451121789No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA21040.19176504043107032No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT20870.19021560807017288No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGAGACCAGGGT20790.18948646342975056No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT20640.18811931722895872No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA20610.18784588798880034No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT20170.18383559246647757No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA20090.18310644782605526No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT19170.17472128446119858No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT18900.17226042129977323No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAACGACGGCGGT18860.1718958489795621No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA18310.16688297957665862No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT17860.16278154097428307No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACAATGACCAGGGT17740.1616878240136496No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT17620.1605941070530161No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT17520.1596826762524882No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGT17490.15940924701232984No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACTAGGGT17410.15868010237190752No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT17150.15631038229053498No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA17130.15612809613042938No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGT17080.15567238073016545No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACTAGGGT17050.15539895149000707No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT16670.15193551444800105No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGAGACCAGGGT16380.14929236512647015No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT16020.1460112142445697No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGT15960.14546435576425296No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA15920.14509978344404179No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGT15790.14391492340335552No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGTGT15680.14291234952277485No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGACACGGTGACCAGGGT15600.1421832048823525No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT15280.13926662632066322No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT15210.13862862476029372No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT15100.13762605087971302No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA14950.13625890467892116No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACGAGGGT14900.1358031892786572No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA14890.1357120461986044No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGC14750.13443604307786536No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT14580.13288661071696795No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCGTGGT14370.13097260603585933No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT13820.12595973663295587No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGT13760.12541287815263913No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT13650.12441030427205846No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACTAGGGT13530.12331658731142497No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCATAGT13460.12267858575105543No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACTAGGGT13060.11903286254894382No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT12760.11629857014736013No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT12690.1156605685869906No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACGAGGGT12690.1156605685869906No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGT12680.1155694255069378No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT12550.11438456546625154No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGT12540.11429342238619875No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA12290.112014845384879No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGACACGGTGACCAGGGT12260.11174141614472062No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGTGT11950.10891598066308415No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGT11800.10754883446229227No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGT11640.10609054518144764No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT11480.104632255900603No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA11350.10344739585991672No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCGTGGT11350.10344739585991672No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT11230.10235367889928325No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCAGGGT11230.10235367889928325No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGAT11190.10198910657907209No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT11170.10180682041896652No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA11150.10162453425886092No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT11070.10089538961843862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)