FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689124
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT302274.386293323117465No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA264183.8335626099221622No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT230133.339457049819771No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC182842.65322351274952No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA179682.6073681949837764No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA174962.5388754418653248No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA157722.288702758864878No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC142062.0614577347473024No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA126981.842629193004452No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGATGAGACAATAAGGAGGGT103861.5071307921361032No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA96241.3965556271440263No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT91611.3293688799113077No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA84621.2279357561193631No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGATGAGACAATAAGGAGGGT82961.2038472031158398No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT70271.0197003732274599No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA68640.996047155519181No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTAACCATTGT53140.7711239196429089No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT50850.7378933254392533No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT41610.603810054503979No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA40790.5919108897672988No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTAACCATTGT40750.5913304427069729No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT39160.5682576720590199No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT38780.5627434249859241No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT38490.5585351837985616No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA38250.5550525014366064No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA36150.5245790307694986No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGT35710.5181941131059141No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT34870.5060047248390711No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT34130.4952664542230426No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGAAGACGGTGACCGTGGT33580.48728530714356194No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA31760.4608749658987352No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT30980.4495562482223808No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT29110.4224203481521468No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGT28880.41908277755527307No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT28200.40921517752973335No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT28180.4089249539995705No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT27620.40079869515500843No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT26320.38193416569441785No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT26060.3781612598022997No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT25300.36713276565610836No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT24650.35770050092581307No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT23660.34333443618274795No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGTT23170.3362239596937561No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA22700.32940370673492725No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT21920.3180849890585729No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGGAGT20190.29298065369947934No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCAGGGT20020.2905137536930944No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGAGCAGGGT19440.2820972713183694No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT19390.28137171249296206No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT19370.28108148896279916No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGATGACCAGGGC19070.2767281360103552No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT19050.2764379124801922No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT18770.2723747830579112No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT18730.27179433599758535No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACAGTGACCATTGT18530.26889210069595604No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGATCACCAGGGT17910.25989517126090517No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA17630.2558320418386241No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGTT17540.2545260359528909No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA16810.2439328771019439No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT16150.23435550060656715No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC15960.23159837707001932No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA15720.22811569470806417No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGATCACCAGGGT15560.22579390646676067No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGGAGT15360.2228916711651314No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGAGCAGGGT15350.2227465594000499No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA15240.2211503299841538No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCAGTGT15170.22013454762858353No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCAGGGT14860.2156360829110581No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT14800.2147654123205693No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT14780.21447518879040636No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGATGACCAGGGC14490.21026694760304387No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGT14470.20997672407288095No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTGGT14380.20867071818714772No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACAGTGACCATTGT13880.20141512993307448No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGGGGAAACGGTGACCGTGGT13570.196916665215549No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA13490.19575577109489728No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA13040.18922574166623132No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA12620.18313104753280976No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT12600.18284082400264684No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC11920.17297322397710715No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC11920.17297322397710715No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCAGTGT11850.1719574416215369No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA11680.169490541615152No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT11670.16934542985007053No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACAGTGACCATTGT11450.16615297101827828No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT11260.16339584748173042No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT11250.16325073571664897No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTATCACAGTGA11220.16281540042140458No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGAT11010.1597680533546938No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT10970.15918760629436793No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGATGGCGGT10500.15236735333553902No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA10480.1520771298053761No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGGGGAAACGGTGACCGTGGT10470.15193201804029463No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGT10400.15091623568472437No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA10350.15019067685931706No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAC10000.14511176508146573No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT9890.14351553566556963No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGTAT9770.14177419448459205No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATG9340.13553438858608902No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC9040.13118103563364505No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGAT9040.13118103563364505No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC8870.1287141356272601No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA8830.12813368856693425No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACAGTGACCATTGT8780.12740812974152693No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGATGACGAGGCT8770.12726301797644546No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGATCAGGGT8770.12726301797644546No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCCGATGAGACAATAAGGAGGGT8760.12711790621136398No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT8670.1258119003256308No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGAGGT8550.1240705591446532No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT8250.11971720619220926No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAC8250.11971720619220926No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA8080.11725030618582433No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA7960.11550896500484673No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGATGGCGGT7940.1152187414746838No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAATGT7930.11507362970960235No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGC7890.11449318264927646No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA7780.11289695323338034No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGTAT7660.11115561205240276No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCCGATGAGACAATAAGGAGGGT7630.11072027675715836No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA7630.11072027675715836No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT7620.1105751649920769No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATG7570.10984960616666957No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA7550.10955938263650664No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGT7490.10868871204601785No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCAGGGT7420.10767292969044759No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT7240.1050609179189812No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGAC7210.10462558262373681No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGATCAGGGT7180.1041902473284924No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC7020.10186845908718896No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGAGAGACGGTGACCATTGT6970.10114290026178162No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGAGGT6920.10041734143637429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)