FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873737
Sequences flagged as poor quality0
Sequence length251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA18823910.04618044047804No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT1551108.278109467870891No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT1026025.475795162287984No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA973745.196780551379409No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA898154.793362142072233No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA852484.549624627148847No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA817824.364646692678855No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT801504.27754802301497No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT673513.5944745714046316No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT490312.61674930900121No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT418702.2345718742811824No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA255871.365559841108971No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA253221.351416981145166No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA238711.2739781516829738No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA169900.9067441161699854No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA154340.8237015120051533No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA147100.7850621511983806No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT138990.7417796627808492No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA132220.7056486582695437No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT105040.5605909473954989No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA91400.48779524554406517No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT91030.48582058207742074No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA88350.47151761426496885No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA80480.4295159886366123No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA80140.4277014330186147No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT75430.4025645007810595No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA73560.39258444488207256No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA73210.3907165199811926No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT71190.37993592483897154No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT70080.37401193443903813No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA68920.36782109762469334No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA65320.3486081557870715No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA62060.3312097695674473No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT58630.3129041055388243No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA56880.3035644810344248No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA56790.3030841574884842No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA55730.29742701350296225No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT54960.2933175787210265No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA51740.2761326696329314No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT49830.26593913660241536No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC48940.26118927042589224No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA47240.25211649233590416No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT46380.2475267340080278No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT43200.23055530205146188No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA42800.22842053073617055No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA41980.22404424953982338No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA39200.20920758889854874No Hit
GNCAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT38620.2061121704913763No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT37240.1987472094536213No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGACCCTGCT32250.17211593729536218No Hit
GANAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT32230.17200919872959758No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT32030.17094181307195194No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTG30590.1632566363369032No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT29530.1575994923513812No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT29300.15637199884508873No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA23220.12392347485266075No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTG22540.12029436361666551No Hit
GNCAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT22170.11831970015002105No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT20500.10940702990867983No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA20460.10919355277715069No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA19720.10524422584386176No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT19050.10166848389074881No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)