FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244789
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1237949.94497862689982No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT785536.310547410042987No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT603624.8491752417477985No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT290582.334371528026035No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT233211.8734902059706504No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT191841.5411447241259362No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT147541.185261116542643No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA143371.151761463187737No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA130701.0499771447209125No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT129991.044273366811564No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT129171.0376859050007672No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA127791.0265996887825968No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA109710.8813541893445396No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT105490.8474528614889753No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT103540.8317875559632999No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT101440.8149172269356494No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA94510.7592451411444028No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA85940.6903981317315625No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT78630.6316733197353126No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT66840.5369584724800749No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT63250.5081182433328059No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA62750.5041014983262223No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA62090.4987993949175322No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA61090.4907659049043653No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT59580.4786353349844833No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT59520.47815332558369333No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA58680.4714051939726331No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT54930.44127960642325725No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT53060.42625698009863516No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA50120.4026385194599245No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT49280.3958903878488644No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT48810.3921146475426759No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA48350.38841924213661916No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA48320.3881782374362241No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT46950.37717235611818545No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT46600.37436063461357705No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT44270.35564260288289823No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT42780.34367270276327955No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT42580.3420660047606462No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT42520.3415839953598562No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT39220.3150734783164054No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT38830.31194041721127036No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA38290.30760233260416026No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT37440.30077386609296836No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT37220.2990064982900717No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT36940.29675712108638497No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT35350.28398387196544955No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT34420.27651272625320433No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC34220.274906028250571No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT33900.2723353114463576No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT31440.252572926013967No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC30500.24502144540159013No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA30470.24478044070119515No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT29420.2363452761873699No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT28720.2307218331781531No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC28240.22686575797183298No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCACAAC27950.22453604586801457No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT27580.22156365456314284No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT27380.21995695656050945No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA26420.21224480614786922No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGT26150.21007576384431417No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGT25720.20662136313865243No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT23720.19055438311231862No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23630.18983136901113362No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC23220.18653763810573518No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCACAAC23130.18581462400455018No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGT22430.18019118099533332No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGT22020.1768974500899349No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGAGAGCAGGGT21320.1712740070807181No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT21120.16966730907808472No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT21090.1694263043776897No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGAGACCAGGGT20900.167899941275188No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGT20440.16420453586913122No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACACTGACCAGGGT20080.16131247946439115No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT18770.15078860754714252No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18680.15006559344595752No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18600.14942291424490417No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACAAGGGT17850.14339779673502898No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGT17620.14155009403200058No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGAGACCAGGGT17400.1397827262291039No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT17210.13825636312660217No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGAGAGCAGGGT16860.13544464162199377No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT16680.13399861341962374No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT15700.12612579320672018No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACACTGACCAGGGT15410.12379608110290177No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT15320.12307306700171676No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGT14560.11696761459170993No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT13670.10981780847999138No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT13640.10957680377959639No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT13560.10893412457854304No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACCACGACGAGGGT13400.10764876617643633No Hit
GACAGCGACCTCGGGTGGGAGCACCTTGTTCAGGTCCTCTGTCACAGTGA13230.10628307287419796No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACAAGGGT13170.10580106347340794No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT12570.1009809694655078No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT12540.1007399647651128No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA12510.1004989600647178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)