FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800683
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA20297011.271834076292162No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1207966.708343445237168No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT911555.062245825611726No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT393822.187059021493511No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT333361.8512975354351653No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT310801.7260117411004603No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA303461.6852494303550376No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA262721.4590019453729501No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT257611.4306238244044065No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA239841.3319390475725046No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT228331.2680188572891509No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA184361.0238337341997452No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT175350.9737971647424893No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT166130.9225943711358412No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA159160.883886836272681No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA143690.7979749906007886No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT143430.7965310940348744No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT132930.7382198865652644No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA132350.7349988865336097No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT113970.6329265062201398No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT106500.5914422471917601No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT100220.5565665916766027No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA97080.5391287639190241No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA96990.5386289535692845No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA96760.5373516604532835No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT89980.4996992807729067No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT89520.4971446945409048No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT78680.4369453146389453No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT76540.42506093521180577No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT76490.42478326279528383No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC74840.4156200730500594No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT71590.3975713659761324No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT70080.38918565899716945No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA69640.38674214173177623No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT68920.3827436589338601No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT65710.36491708979315074No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT62020.3444248654538306No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC61300.3404263826559144No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT58880.3269870376962519No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT58450.32459905491416313No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT57840.3212114514325953No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA57270.31804598588424504No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA56140.3117705892708489No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT55590.3087161926891074No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT52330.2906119511318761No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT49340.2740071406238633No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA48100.26712086469411883No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT47910.2660657095113354No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGT47050.2612897439471578No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT45960.25523648526697923No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT45910.2549588128504573No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC45170.25084926108593236No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA45110.25051605418610606No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT44920.2494608990033226No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT40860.22691389878174004No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT40580.22535893324921713No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGT39670.22030529526851755No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGT39310.21830605386955948No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT38320.21280814002242482No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT35750.19853577781319642No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT35450.19686974331406473No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC34250.19020560531753786No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT33430.1856517776865778No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGT32770.18198650178848805No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT32400.17993172590622558No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT31410.1744338120590909No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT31190.1732120534263943No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGT29620.164493139547605No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT29080.1614942774491679No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT28850.16021698433316692No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT28410.15777346706777373No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGT28290.15710705326812105No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGATT27810.1544413980695103No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGCGACTAGGGA27200.15105379458794246No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT26580.14761065662307027No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT26380.14649996695698242No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA25970.14422305314150244No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA25520.14172400139280483No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT25460.1413907944929785No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGT25270.14033563931019508No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA24630.13678143237871407No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAAACAGTGACCGGGGT24340.13517093236288674No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT24150.1341157771801033No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT23980.13317169096392867No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT23500.13050603576531794No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGT23180.12872893229957744No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT23050.12800698401662036No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGAGACCAGGGT22820.12672969090061936No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT22810.126674156417315No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT22260.12361975983557351No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA22060.12250907016948569No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTTCCTCTGGGA21890.12156498395331104No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT21890.12156498395331104No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGATT21800.12106517360357154No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGCGACTAGGGA21160.11751096667209054No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGCCACGGTGACCCGGGT21120.11728882873887297No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACGAGGTG21050.11690008735574225No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCACAAC20790.11545619078982808No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT20730.11512298389000172No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA20570.1142344321571315No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT20470.11367908732408757No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA20380.11317927697434807No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCATAGGAGACGGTGACCCGGGT20170.11201305282495587No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAAACAGTGACCGGGGT19870.11034701832582415No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA19520.10840331141017047No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT19500.1082922424435617No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTAGT19490.1082367079602573No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA19470.10812563899364853No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT18990.10545998379503778No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18680.10373841481260165No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCTGTGT18570.10312753549625336No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT18190.10101722513068652No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGT18170.10090615616407773No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACGAGGTG18090.10046188029764261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)