FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436528
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA14936910.397917757259169No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT1436189.997577492398339No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1181168.222324939019636No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT1132407.88289542563737No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT918156.391452168005079No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA196011.3644704454072598No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA163011.1347498969738146No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA136080.9472840069946427No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT124310.8653503447200472No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA122910.8556046244834768No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT121160.8434224741877638No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT108950.7584258712673891No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT106030.7380990833453994No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA102230.7116464141318513No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT99750.6943825668556408No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT98780.6876301749774456No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT93660.6519886838265596No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA91570.6374397157591081No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT89660.6241437688649298No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA87030.6058357372776584No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT84000.5847432141942239No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT82940.577364311729392No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT76760.5343439181136741No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT75880.5282180368221155No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT75120.5229275029794059No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA73370.5107453526836929No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT72660.5058028802780036No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT69920.48672911352928727No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA68450.47649610728088837No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT61070.42512223917668157No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT58690.4085545147745119No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT57850.4027070826325696No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT57140.3977646102268803No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT55370.3854432353563592No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT54600.3800830892262455No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT45700.3181281534366194No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT43900.30559794170388604No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT43270.30121236759742936No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT42480.2957129968925075No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT40510.28199937627390487No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT34880.24280765846541105No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT34010.23675138946125657No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGCTGAGGACGGAGGA33590.23382767339028546No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT31620.22011405277168283No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA30290.21085561854694096No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT29540.2056346969916354No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT28950.20152757203479502No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT28860.20090106144815836No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT27330.19025038147533496No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT26870.18704821625474757No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT26380.18363721417194792No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA25690.1788339663410668No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGCTGAGGACGGAGGA24820.1727776973369123No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA24660.17166390073844714No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA24190.16839212323045566No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT24070.16755677578160677No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23830.16588608088390897No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT22940.15969058730494637No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT21630.15057137765501263No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT21110.1469515387100008No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT20610.14347092433979705No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC19870.13831961507189558No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT19500.13574396043794482No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT19450.13539589900092444No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT19250.13400365325284297No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT18760.13059265117004334No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT18560.12920040542196184No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA18450.12843467026051703No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGCTGGATCCT17560.12223917668155442No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA17230.11994197119721997No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC16520.1149994987915307No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT16390.11409453905527772No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGAGGACCGTGGT16280.1133288038938329No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT15900.11068353697247807No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT15630.1088040052125681No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGT15460.1076205963266988No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCATTGT15330.10671563659044585No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT15050.10476649254313178No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT14960.1041399819564951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)