FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258496
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1241379.863916929414158No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT1167479.276708070585842No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1024308.139080299023597No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT922807.332562042310823No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT803596.385320255288852No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA190871.5166516222538649No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT134321.0673057363710334No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT115510.9178416141171684No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA113090.8986123118388935No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT106490.8461687601708706No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT98380.7817267595606183No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT92630.7360373016680227No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT89180.7086236269324654No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA88230.701074933889341No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT88090.6999624949145646No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA86430.6867721470707893No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT79610.6325804770138324No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT78760.6258263832384052No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA78680.6251907038242474No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT75760.6019884052074858No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT74070.5885596775834011No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT70250.5582059855573638No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA70200.5578086859235151No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT69540.5525643307567127No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT67910.5396123626932465No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT66920.5317458299430432No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA60780.48295743490642795No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA60430.4801763374694874No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT60180.4781898393002441No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT58560.4653173311635476No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT57750.4588810770951994No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA57180.4543518612693247No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT55520.4411615134255492No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT53130.42217059092758347No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT52960.42081977217249794No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT51650.41041052176566317No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT49230.39118121948738815No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT46820.3720313771358828No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT43120.3426312042310822No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT42120.334685211554109No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCATTGT41390.32888463689991865No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT41220.32753381814483323No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA40450.32141540378356387No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT39730.3156942890561432No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT35030.27834812347436944No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT35000.2781097436940602No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCATTGT32950.26182045870626525No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT32340.25697340317331163No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA31670.2516495880797396No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT27340.2172434397884459No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT26390.20969474674532143No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT26150.20778770850284783No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCCAGGAGACGGTGACCGTCGT25770.20476823128559807No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT25060.19912657648494708No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA24940.19817305736371033No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT24850.19745791802278276No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCCAGGAGACGGTGACCGTCGT21760.17290480065093572No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT20960.1665480065093572No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT20630.16392582892595606No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT20440.16241609031733117No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT20310.16138311126932464No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT20240.16082689178193654No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT19350.15375495829943042No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT19010.15105332078925957No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT17150.1362737744100895No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT17070.13563809499593163No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCCGGGT17020.135240795362083No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT16740.13301591741253052No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA16480.13094995931651748No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT16340.12983752034174126No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT16320.1296786004882018No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA16070.1276921023189585No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGT15170.12054070890968266No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA14820.11775961147274207No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGATGAGGAGACGGTGACCAGGGC14770.1173623118388934No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT14290.1135482353539463No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATG13410.10655576179820993No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCCGGGT13290.10560224267697316No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT13050.10369520443449959No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT12750.10131140663140764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)