FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305159
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT1251579.589406348192059No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT921547.060748920246499No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA890666.824149394824691No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT792156.069375455404284No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT578744.434249007209083No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT146871.1253035070822788No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT114050.8738398923043093No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA112800.8642625151418334No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT103550.793389924139511No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA93230.7143190982861092No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT90980.6970798193936524No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT80630.6177791364883513No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA80490.6167064702461539No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT78470.6012294287515927No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT77240.5918052896237164No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT75020.5747958677831589No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA74990.5745660107312596No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT70250.5382485965311506No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT68750.5267557439361794No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT67750.5190938422061986No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT66400.5087502748707246No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA61160.4686019098056252No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT60510.4636216736811377No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT59730.4576453903317527No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA58730.44998348860177184No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT57480.4404061114392959No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT56380.43197801953631704No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT54970.4211747380970441No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT52570.4027861739450902No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA51250.3926724636615156No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGT50990.3906803692117206No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT50860.38968432198682307No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT49990.3830184674817398No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT49180.3768123270804553No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT48790.37382418540576284No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA46450.35589533535760776No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT45900.3516812894061183No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT45640.34968919495632333No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT44270.33919238958624964No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA41600.31873511196720095No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGT41380.31704949358660517No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGT39280.3009594999536455No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT38990.29873754845195105No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC38140.2922249319814674No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT37610.2881641240645776No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT37170.28479288730338603No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT35820.27444931996791194No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGT33100.2536089472623642No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT32530.24924166327627514No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGT31640.24242257073659224No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT31160.23874485790620148No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT28240.21637210485465755No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT28050.21491634352596123No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC26940.20641163260568252No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT26250.20112492041199576No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT25630.1963745413394077No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA24780.18986192486892403No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT24660.18894249666132631No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT24570.18825292550562805No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA24220.18557125990013476No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGT24060.18434535562333784No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA22350.1712435036650707No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT22250.17047731349207262No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT21390.16388807800428912No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT20650.15821827072410335No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGT19680.150786226046022No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT19680.150786226046022No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT19470.149177226682726No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT18800.14404375252363888No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT18750.14366065743713985No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGT18750.14366065743713985No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT17770.13615199374175868No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA17390.13324047108436596No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT17220.13193794779026924No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT16620.12734080675228077No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGCAGAGACGGTGAGCACTGT16470.12619152149278362No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT15760.12075157126449727No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT15510.11883609583200208No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGCGACGGTGACCAGGGT15450.11837638172820322No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA14590.11178714624041974No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTTGT14160.10849252849652802No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT13950.10688352913323204No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGT13930.10673029109863244No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGT13930.10673029109863244No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGT13890.1064238150294332No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCAGT13600.10420186352773876No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT13600.10420186352773876No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC13310.10197991202604434No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGT13160.10083062676654721No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGC13120.10052415069734799No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT13070.10014105561084895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)