FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813199
Sequences flagged as poor quality0
Sequence length251
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA32184739.57788929892929No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA565486.953771463073615No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA292933.602193313075889No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA228422.8089065530085504No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA187992.311734274144459No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA154701.902363382148773No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG140111.7229485033798615No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG113001.3895737697660722No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT98171.2072075838755336No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT81290.9996323163210972No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA69400.8534196426704903No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA64880.7978366918798474No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG54670.6722831680806297No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCC53990.6639211312360197No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA52340.6436308947748337No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA52060.6401877031329355No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT50430.620143408931885No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA46380.5703401012544286No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG44940.5526322585246661No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA42180.518692226625955No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA37960.466798409737346No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT36120.44417172180487186No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT35770.43986773225249914No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT32750.40273045097202526No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA32150.39535218316795767No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT28000.34431916418982317No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA27150.33386661813406066No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA26660.3278410327607388No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT26310.32353704320836596No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA24680.30349274900731554No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA21840.26856894806806203No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT20620.2535664701997912No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC19170.23573565633996105No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA17830.21925752491087666No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT16500.2029023646118601No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA16280.20019699975036861No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA15600.1918349629057586No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA14440.1775703118178945No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC13480.1657650833313863No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTGA12730.15654224857630175No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA12240.15051666320297982No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA11980.1473194138212172No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG11460.1409249150576919No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA11100.1364979543752513No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG9800.12051170746643812No Hit
GACAGCGACCTCGGGTGGGAACACGTTGTTCAGGTCCTCGAGCACTGTCA9660.11879011164548899No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA9120.11214967062182811No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG9090.11178075723162473No Hit
GACAGCGACCTCGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG8960.11018213254074341No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACTGTCA8420.10354169151708253No Hit
GANAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG8160.10034444213531989No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)