FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001880436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899973
Sequences flagged as poor quality0
Sequence length251
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT625936.954986427370599No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT485745.39727302930199No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT348123.8681160434813044No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC291953.2439862084751434No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT281593.1288716439270954No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC219142.4349619377470213No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA185822.0647286085249226No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG154051.7117180182072127No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG146991.6332712203588329No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA130161.446265610190528No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA128901.4322651901779275No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG127001.4111534457144825No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG122971.3663743245630702No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA118201.3133727345153687No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA107051.18948012884831No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT101111.123478148788908No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT99161.101810832102741No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA97271.0808102020838402No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT89580.9953631942291602No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA89360.9929186764491824No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTA81010.9001381152545687No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT80130.8903600441346573No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT76740.8526922474340898No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT70260.7806900873692877No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTA61810.6867983817292297No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA61680.6853538939501519No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACAGTGGT58680.6520195605868176No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT55220.6135739627744388No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT54810.6090182705481164No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT52110.5790173705211156No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA50640.5626835471730819No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCCGCTGCTCTGCCCTGGTC48610.540127314930559No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA45600.5066818671226803No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACAGTGGT45030.5003483437836468No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTCCCCCGTGCTGGCTCCGCTT44310.49234810377644667No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT43470.48301449043471306No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT42960.47734765376294624No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT42480.4720141604248128No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA42250.4694585282002905No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT40270.4474578681804899No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTAAAGAGACGGTGACCATTGT39340.43712422483785623No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCCGCTGCTCTGCCCTGGTC39080.43423524927970064No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT38680.42979067149792277No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT38310.4256794370497781No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTCCCCCGTGCTGGCTCCGCTT35490.39434516368824396No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT32330.3592329992121986No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT31830.3536772769849762No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTAAAGAGACGGTGACCATTGT31180.3464548380895871No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT30720.3413435736405426No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT30430.33812125474875354No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT30220.3357878514133202No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT29510.32789872585066443No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCAC27970.3107871013908195No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA27870.30967595694537503No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC26380.29311990470825233No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA26090.28989758581646335No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCCAGAAAGCTTGCAGGACCGG24780.27534159358114074No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT24530.2725637324675296No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT24360.27067478691027397No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC24170.2685636124639295No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT23740.26378569134851826No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT23460.2606744869012737No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT23090.25656325245312916No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAAAGACGGTGACCAGGGT22110.24567403688777328No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC20320.2257845513143172No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCCAGAAAGCTTGCAGGACCGG20300.2255623224252283No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCAC20190.2243400635352394No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC20150.2238956057570616No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT19740.21933991353073923No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC19290.21433976352623912No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT18640.20711732463085003No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT18220.20245051795998326No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAAAGACGGTGACCAGGGT17930.19922819906819425No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT17050.1894501279482829No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA16970.1885612123919273No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGATGGTGACCGTGGT15410.1712273590429935No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCTGCCCTGGTC15330.17033844348663793No Hit
GANAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC15320.1702273290420935No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT14670.1630048901467044No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT13840.15378239124951526No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA13430.1492266990231929No Hit
GNCAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC13270.14744886791048176No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG13250.14722663902139288No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG12680.14089311568235935No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGATGGTGACCGTGGT12660.14067088679327047No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA11680.12978167122791462No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT11650.1294483278942813No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCTGCCCTGGTC11610.12900387011610348No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA11350.12611489455794786No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG10860.12067028677526992No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGT10640.1182257689952921No Hit
GNCAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC10590.11767019677256985No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA10460.11622570899349202No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG10310.11455899232532532No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA10230.11367007676896974No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAACGAGACGGTGACCAGGG10180.1131145045462475No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG9580.10644763787358066No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT9350.10389200564905834No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG9170.10189194564725831No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT9100.10111414453544718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)