FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227428
Sequences flagged as poor quality0
Sequence length251
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA787076.412351681727971No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT531114.327015515370352No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT445543.62986668057108No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT398043.2428786046920877No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT349992.851409614250286No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA306062.49350674744262No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA264342.1536090100600607No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC197921.612477473220425No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA160401.3067976288629557No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG156661.276327409835852No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG150571.2267114649494715No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC146371.1924935719243817No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA136251.1100447439686891No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG129461.0547258169114604No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG119790.9759431917798844No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA114180.9302378632392286No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT103310.8416786972433414No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA101720.8287247805981288No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT79800.6501399674767073No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT77900.6346604444415477No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT77380.6304239434003461No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT74250.6049234659792673No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA68630.5591366662647421No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA68280.5562851751793181No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT64990.529481158976331No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACAGTGGT61660.5023512580778668No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT61280.499255353470835No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT59280.4829611186969826No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT59050.4810872816979896No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA56420.4596603629703738No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT56040.4565644583633419No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA54440.44352907054426No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA52070.424220402337245No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT50470.4111850145181632No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACAGTGGT50470.4111850145181632No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT45030.36686469593328486No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA44480.3623837813704755No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT42050.34258628612024494No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA41920.3415271608599445No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA41480.337942429209697No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA39000.31773757809012015No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT37740.3074722101825932No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA37270.3036430650107379No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT35920.2926444565383876No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT34960.2848232238469385No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT33540.2732543171575033No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT30140.24555411804195437No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT29850.24319145399974582No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT29760.24245821343492246No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC28250.23015606618066398No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTCCTGAAGTTCCCTTTTCTTC27050.22037952531635258No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTAAAGAGACGGTGACCATTGT26590.21663185131836651No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA25990.21174358088621084No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT25530.2079959068882248No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT24970.20343352115154617No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT24290.19789348132843637No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTA23960.19520493259075072No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA22830.1859986899435242No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT22650.18453220881387747No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC21910.17850334194755213No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT21830.177851572556598No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA20330.1656308964762088No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTCCTGAAGTTCCCTTTTCTTC20330.1656308964762088No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT19940.16245352069530758No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGCGCCGCCACTGGACGGA19750.1609055683917916No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTAAAGAGACGGTGACCATTGT19680.1603352701747068No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT19260.1569134808721978No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT18900.15398051861290438No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT18370.14966254639783352No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC17600.14338926600990037No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTA17460.1422486695757307No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT16820.13703451444809797No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGCGCCGCCACTGGACGGA16760.1365456874048824No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT16440.13393860984106604No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA16310.13287948458076562No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACGGGGA15760.12839857001795624No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT15670.12766532945313291No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT15450.12587296362800915No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCGTGGT15280.1244879536722317No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT14840.12090322202198418No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA14500.11813320211042928No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA14170.11544465337274365No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC13770.1121858064179732No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCAC13760.11210433524410394No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG13710.11169697937475763No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG12890.10501634311747816No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC12890.10501634311747816No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACCGTGACCAGGGT12870.10485340076973966No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA12730.10371280433557No Hit
GACAGCGACCTCGGGTGGGAACACGCTTTTCCCGGCCCCGTGTGGACTTT12610.10273515024913885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)