FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380718
Sequences flagged as poor quality0
Sequence length251
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA25344918.356318958686714No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT487543.531061375313424No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT380082.7527706599030357No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT296532.1476507150627424No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA269681.9531866753384832No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT264491.9155975369336824No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT240901.7447444011014561No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA234221.6963637759484558No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT226631.6413923770096428No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT213651.547383317954861No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA204151.4785785366743969No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA177831.2879530794847318No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT176631.279261949217726No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA172811.2515951845344235No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG170641.2358787239682543No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC162351.1758374990403544No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA159411.1545442298861897No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT150071.0868982659746595No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA137160.9933961895187866No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG136980.9920925199787357No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG125520.9090922259288283No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA121640.8809909047321756No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC121230.8780214352242818No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT118310.8568730182412339No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG107210.7764800632714284No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGAT106440.7709032546834328No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT104770.7588080983951828No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA100400.7271578990061692No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGAT84950.6152595968184669No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT83890.6075824317492783No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA81610.5910692842419668No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA73580.532911137538585No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA67930.4919903991980984No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA66640.4826474341610669No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT62680.4539667042799471No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT60080.4351359220347674No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGC57280.41485661807842006No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA56410.4085555486348407No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA55990.40551365304138864No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA55600.4026890357046117No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGTGT54220.39269423589755476No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT51690.37437043625128374No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTCCTGAAGTTCCCTTTTCTTC49170.35611906269057114No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGC46960.3401128977821684No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT46360.3357673326486654No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA44620.3231651937615067No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGT44530.32251335899148126No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGTGT43670.3162847156334603No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA42090.3048413941152357No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT40420.29274623782698567No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA38530.2790577076564512No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTCCTGAAGTTCCCTTTTCTTC37430.27109083824502905No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGT37190.2693526121916278No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT35130.25443283856660087No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCACTGT34630.2508115342886817No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT33210.24052703013939125No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT32980.23886123017154842No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT32670.23661602151923855No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT32210.2332844215835529No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT31310.2267660738832984No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGTGGT30290.2193786131563433No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGT29640.2146709175950484No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCACTGT29030.210252926375987No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT27770.2011272395956307No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT27420.19859232660108728No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA27250.19736108314659476No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT26710.19345007452644203No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTA26160.18946663982073095No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGTGGT25290.18316557037715162No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT24570.177950892216948No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC24000.17382260534012015No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT23820.17251893580006925No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT23160.16773881415321595No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT22230.16100318819628628No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA22200.16078590993961112No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT20710.149994423191412No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC19390.1404341798977054No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC19380.14036175381214702No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT18960.1373198582186949No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT18870.13666802344866946No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT18610.1347849452241515No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCAC18530.13420553653968442No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT16890.12232765850810955No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA16880.12225523242255117No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT16730.1211688411391754No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA16670.12073428462582511No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGACACGGTGACCAGGGT16160.11704055426234757No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC16070.11638871949232212No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT15780.11428836301112899No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA15500.11226043261549426No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC15480.11211558044437749No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG15440.11182587610214396No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT15330.11102918916100174No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGATCAGGGT14700.10646634577082359No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACAGTGACCAGGGT14560.10545238057300622No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT14320.10371415451960501No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT14310.10364172843404663No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA14180.10270018932178765No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT14150.10248291106511251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)