FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632538
Sequences flagged as poor quality0
Sequence length251
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA31789619.472502324601326No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA874805.358527642235587No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT558753.422584956674821No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT428742.6262175826841396No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA413092.5303545767387954No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT306631.8782411190428645No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC291371.7847670314565418No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA289711.7745988148514766No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG249191.5263963227808481No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT241731.4807006023749525No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT238561.4612829839182917No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA219121.3422045918686119No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC218361.3375492637843651No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG197661.2107528278055395No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA196771.2053011936016191No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT194961.1942141622430842No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT181261.1102957480928468No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA155980.9554448349747449No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT135410.829444705115593No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA126140.7726619533511624No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA124370.7618199392602194No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT119720.7333366819026571No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGAT113010.6922350352641102No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA108790.6663857135331612No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA102230.6262028816480841No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG96780.5928192789386831No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGT96260.5896340544599881No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGAT90220.5526364470536062No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT83590.5120248349502431No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCG82470.505164351457669No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTG79820.4889319574797034No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA79820.4889319574797034No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC69650.4266363171944542No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT63640.38982247273876625No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA62270.38143063132374255No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA60760.37218122947214705No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAAC56460.3458418732060142No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTA50490.3092730460179181No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT49120.30088120460289436No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGT48520.29720594558901536No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGACACGGTGACCAGGGT45770.28036100844206996No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT45670.2797484652730901No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA44330.27154038680876036No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGCACCACAGGCTCTGGCTGA43860.26866143391455516No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA42800.26216847632336887No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGT42440.2599633209150415No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGC41190.25230653130279357No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTA39620.24268960354981017No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGT39290.24066821109217673No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA37260.2282335847618861No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGA36520.2237007653114353No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGACACGGTGACCAGGGT36460.22333323941004743No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT36310.22241442465657768No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT36310.22241442465657768No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT35310.2162889929667793No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA35190.2155539411640035No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGCACCACAGGCTCTGGCTGA35000.21439010914294185No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCGTGGT32680.2001791076226097No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCAC32560.19944405581983393No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGC31930.19558503385526094No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA31800.19478872773558717No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT27140.1662442160611269No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCACTGT27120.16612170742733096No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT27110.16606045311043297No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA26910.1648353667724733No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC26470.16214017682896203No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT25200.15436087858291814No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT25000.15313579224495846No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGTAACGGTGACCAGGGT24690.15123690842112097No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT24380.1493380245972835No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT24220.14835795552691575No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCAC23150.14180374361883152No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT22980.14076242023156582No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT21540.13194179859825622No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCACTGT21240.13010416909131672No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG21160.12961413455613285No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA20540.12581636690845788No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC20410.12502006078878408No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT19910.12195734494388492No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT19320.1183433402469039No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGTAACGGTGACCAGGGT19130.11717950822584221No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT19090.11693449095825027No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTC18780.1150356071344128No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT18750.11485184418371884No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGT18730.11472933554992287No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTT18540.1135655035288612No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT17920.10976773588118621No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGATCAGGGT17830.10921644702910438No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA17110.10480613621244957No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGT16980.10400983009277578No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTG16450.10076335129718267No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT16360.10021206244510082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)