FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734750
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT19420011.19469664216746No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1248757.198443579766536No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1198406.9082000288225975No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA853074.917538550223375No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA591693.4108084738434936No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT520833.0023346303501945No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT395332.278887447759043No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA378122.1796800691742324No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA357092.0584522265456116No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT338231.949733390978527No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA333831.924369505692463No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA270041.5566508142383628No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT268941.5503098429168467No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA198481.1441418071768266No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA195501.1269635394149011No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT169200.9753566796368354No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT129040.7438535812076669No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT121990.703213719556132No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT112360.6477013978959504No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA107440.6193399625306241No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC103430.5962242398040063No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT92810.5350050439544604No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA82990.4783974636114714No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT82690.4766681077965125No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT76770.4425421530479896No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA76440.4406398616515348No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA70630.4071480040351636No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC70330.4054186482202046No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA67610.38973915549791033No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT61090.35215448911946967No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA56860.32777057212854877No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA55980.32269779507133595No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA55320.3188932122784263No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT53590.30892059374549646No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT51960.2995244271508863No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA50820.2929528750540424No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGATGAGGAGACGGTGACCAGGGT49350.2844790315607436No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT44510.2565787577460729No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT44260.25513762790027383No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA40710.2346735840899265No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA38680.22297160974203775No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA37680.2172070903588413No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT34660.19979824182158815No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT33740.1944948839890474No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT33500.19311139933708027No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT32470.18717394437238796No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA32320.1863092664649085No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGATGAGGAGACGGTGACCAGGGT32260.18596339530191672No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT31520.18169765095835136No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT30860.1778930681654417No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT30440.1754719700244992No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA29960.1727050007205649No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA29360.16924628909064707No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA28470.16411586683960225No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT26070.15028102031993085No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT25820.1488398904741317No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT25160.14503530768122208No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA24800.14296008070327137No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23770.13702262573857904No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT23250.1340250756593169No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT23070.13298746217034155No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA22230.12814526588845657No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA22140.12762645914396886No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA21650.12480184464620263No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA21200.12220781092376423No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT20530.11834558293702263No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT20180.11632800115290388No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGT20090.1158091944084162No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT20040.11552096843925638No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT19740.11379161262429745No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA19130.11027525580054763No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT18930.10912235192390835No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT18660.1075659316904453No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA17610.10151318633808908No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT17560.10122496036892924No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA17550.10116731517509728No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA17470.10070615362444157No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT17360.10007205649228995No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)