FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555823
Sequences flagged as poor quality0
Sequence length251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT21089513.555205187222455No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1315448.454946353151998No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1214717.807507666360506No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA639584.110878936742805No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT590433.7949689649786644No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA550683.5394771770310633No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT396192.5464978985398723No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT357182.295762435701233No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA291831.8757275088490142No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA255221.6404179652826831No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA241691.5534543453850471No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA231641.4888583084322573No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT223741.4380813241609103No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA201351.294170352283004No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT187141.2028360552582138No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT185811.19428752499481No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT173581.1156796113696739No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA157151.0100763390179988No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT139440.896245909721093No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT127920.8222014972140148No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT123030.7907711866966873No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT117430.7547773750613019No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA111570.7171124221714167No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA110220.7084353425807435No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT100890.6484670814096462No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT90170.5795646419933372No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA84900.5456918942578943No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGAGCCTGGCGGACCCAGTG81370.5230029379948747No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA78730.506034426795336No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT63640.40904395937069965No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA58200.3740785423534682No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGAGCCTGGCGGACCCAGTG54960.35325355133585246No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCCGGAT54880.35273935402677553No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA53780.3456691410269677No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT52950.34033434394529455No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA48880.3141745558460056No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA44890.2885289650557936No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA44490.28595797851040894No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT41400.26609710744731246No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA40590.26089085969290854No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT39340.25285652673858144No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCCGGAT37470.24083716463890814No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT35580.2286892532119656No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA34770.22348300545756167No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT31580.20297938775811902No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA31000.19925145726731125No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGAGCAGGGT29850.19185987094933035No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT29330.18851758844033029No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA27630.17759089562244548No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC27130.17437716244071466No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT27040.17379869046800311No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA26760.17199899988623385No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT26470.17013503464082996No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA25870.16627855482275297No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA25350.1629362723137529No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT23670.15213812882313732No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGAGCAGGGT22700.14590348645057954No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA21420.1376763295053486No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA21310.13696930820536782No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGCGT21020.13510534295996396No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT20880.13420549766907933No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA20130.12938489789648308No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT19630.12617116471475226No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA19470.12514277009659838No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA18390.1182011064240598No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18260.11736553579680979No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT17510.11254493602421356No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA17160.11029532279700197No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT16360.10515334970623265No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC15860.10193961652450183No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA15700.10091122190634795No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT15640.10052557392454026No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA15630.10046129926090563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)