FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237724
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA840006.786650335616018No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT758076.124709547524327No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT563604.5535192013728425No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT512194.138160042141867No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT427073.4504461414661103No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC383043.094712553040904No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA343992.779214105891136No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA290322.345595625519098No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT286912.3180450568947517No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT282732.2842733921294247No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC261512.112829677698744No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA255622.0652423318930553No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT210461.700379082897318No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA155721.2581157026930074No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT147121.1886333302093197No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA104180.8417062285291389No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT101290.8183569196363647No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA88890.7181730337296521No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCGTGGT80180.6478019332258241No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA77780.6284115036954927No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA72880.588822710071066No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT68720.5552126322184914No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA67110.542204885741894No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT65860.5321057036948463No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGT63410.5123113068826329No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT63240.5109378181242344No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA55670.44977717164731396No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCGTGGT54230.4381429139291151No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT50330.4066334659423264No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA46480.37552798523741965No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT46380.3747200506736559No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT44620.36050040235141273No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT42970.34716948204930986No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT41500.33529284396198183No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT40610.3281022263444839No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA40320.32575921610956887No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT36950.29853182131072836No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA36190.29239151862612345No Hit
GANAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT36190.29239151862612345No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGT35600.28762470469991697No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT35300.2852009010086255No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC34370.27768710956562204No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT33870.273647436746803No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC31700.2561152567131283No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA30660.24771273724998466No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA30180.24383465134391835No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT29360.2372095879210551No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA28860.23316991510223603No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT26790.21644566963232517No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT26630.21515297433030303No Hit
GNCAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT26470.21386027902828095No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT26170.21143647533698953No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT26080.21070933422960209No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT25890.20917425855845082No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC25560.20650807449803027No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC25060.2024684016792112No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA24110.19479302332345497No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT24010.19398508875969117No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT23350.18865272063885No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA22420.18113892919584657No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGAGT21710.1754025937931235No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC21670.17507941996761797No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT21650.17491783305486522No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC21550.1741098984911014No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACTAGGGT21060.17015101912865874No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT21030.1699086387595296No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA20930.16910070419576578No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA20910.168939117283013No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT20340.16433389026955927No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA20040.16191008657826786No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT19720.15932469597422366No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGT19300.15593137080641564No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC18950.15310359983324232No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGT18280.14769043825602476No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT18220.1472056775177665No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT17930.1448626672828514No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA17890.1445394934573459No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT17860.14429711308821677No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT17590.14211568976605446No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT17550.14179251594054895No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT17190.13888395151099922No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT17050.1377528431217299No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA16560.1337939637592872No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC16470.13306682265189976No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGTGGAGACGGTGACCAGAGT16090.1299966713095973No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA15780.12749207416192948No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA15730.12708810688004757No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT15180.12264446677934661No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT14720.11892796778603307No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA14600.1179584463095165No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT14290.11545384916184868No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGT14290.11545384916184868No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT14210.11480750151083763No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT13860.11197973053766429No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT13600.10987910067187838No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA13490.1089903726517382No Hit
GNCAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT13270.1072129166114578No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT13200.10664736241682313No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT13160.1063241885913176No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT12970.10478911292016638No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA12910.10430435218190807No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT12840.10373879798727342No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT12810.10349641761814427No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGC12760.10309245033626237No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT12570.10155737466511112No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATG12380.10002229899395988No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)