FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244419
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT17309413.909623687841474No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1081108.687588344440258No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA835826.7165480437055365No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA723985.817815382118081No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA476623.8300604539146383No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA465593.7414247130588656No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT337772.7142787115915135No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA317962.5550879567091145No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA263542.117775443801485No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT232011.8644041918357082No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA198471.5948808239025603No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA165161.3272057080452806No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA161501.2977943924032018No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA151151.2146230489891268No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT148001.1893100314283211No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT111570.8965629743679581No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA100810.8100969207316828No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT94710.7610780613282182No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT83720.6727637556160747No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT82290.661272449231328No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA76460.6144232770473611No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA75270.6048605815243902No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT71010.5706277387278722No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA65590.5270732767661053No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGGTGACCATTGT64450.517912375172671No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA63050.5066621451456463No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA57190.45957189660395736No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT55930.4494466895796352No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT51360.4127227244199904No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAAGAGACGGTGACCATTGT47060.3781684464798432No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA45700.36723965159644784No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT44140.35470368099490607No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT41890.3366229541657593No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA41430.33292645001402266No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA41280.3317210682254128No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA40290.32376554842058825No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA38900.3125956771794709No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA35840.28800588869183125No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT32280.2593981609088257No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA31890.2562641682584403No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA29800.2394691820038106No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT29710.23874595293064474No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT29360.23593339542388858No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT28160.22629034111501029No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA26320.21150432450806358No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA23470.1886020705244777No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA23190.18635202451907276No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA23100.18562879544590688No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT22350.17960188650285794No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTG22210.17847686350015549No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT20940.168271297689926No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT20440.16425335839456004No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA20180.16216402996096974No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTG19490.15661927373336473No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA17370.1395832111210131No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA17090.13733316511560817No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC16320.1311455386007446No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT16220.13034195074167143No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA16140.12969908045441286No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA15080.12118104914823705No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA15040.12085961400460778No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT14910.11981494978781262No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA14300.11491306384746618No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA13570.10904687247623188No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA13550.10888615490441723No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA13380.10752005554399281No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA12940.10398426896407079No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGT12640.10157350538685121No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)