FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488419
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT29316719.6965370638241No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT20211913.579442347887255No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT720034.8375491041165155No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT530423.563647064435485No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA393312.642468283460504No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT371792.497885340082329No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA347302.333348338068783No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA257091.7272690015378733No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT256391.722566024755126No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT252751.6981105454848398No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT206421.3868406678495773No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT190661.2809565048551517No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA186941.2559635425239801No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT171631.153102721747035No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT149891.007041699951425No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT143580.9646477235240882No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA133950.8999482000700072No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT132900.8928937348958862No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGT107260.7206304138821125No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT100740.6768255444199517No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA97970.6582151934367944No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT94650.6359096464100499No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGT75520.5073840094758263No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT67480.4533669618568427No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA61730.4147353668557039No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT59470.39955147038569117No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA52260.3511108095233936No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT52160.35043895569728684No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT45540.3059622324090192No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT45030.30253577789587477No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA43930.29514538580870037No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT39570.2658525589904456No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT37970.25510289777273737No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT34670.23293172151121425No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA33860.22748970551974948No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT32970.22151020646739933No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA30720.20639349537999716No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT30600.20558727078866906No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCATTGT30340.2038404508407915No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA29760.19994369864937225No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT28120.18892529590122137No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT27450.18442387526630605No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA27070.18187083072710036No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA26550.1783771908313452No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT26460.17777252238784913No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT26200.17602570243997154No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA25480.17118835489200285No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGT24750.1662838219614235No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA23810.15996839599601995No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23470.15768409298725694No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA22920.15398889694366977No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT22690.15244363314362422No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA22650.1521748916131815No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA21410.14384390416945766No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCATTGT20720.139208112769321No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT20160.13544573134312313No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT19720.13248957450825338No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT19580.1315489791517039No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT19450.13067556917776513No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTG18940.12724911466462063No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT18240.12254613788187331No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA18030.12113524484704911No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT17560.11797753186434734No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA17020.11434952120337083No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT16940.11381203814248542No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA16520.11099025207283703No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA16050.10783253909013522No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCAAGAGAGGTGGTGCCATG15380.10333111845521993No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)