FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621111
Sequences flagged as poor quality0
Sequence length251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT22111113.639473176111938No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT1396368.613598945414596No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT833095.139006520836636No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT546683.3722552002916517No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT519893.2069981636050833No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA498903.0775190594598394No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT339472.09405771720752No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA279131.7218438465965626No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCATCGT250831.5472722102311316No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA236401.4582591815119383No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT195381.2052228379179464No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCATCGT184481.1379849991764908No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA172501.064085062651478No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT163961.0114051412889062No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT146670.9047498906614043No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT125180.7721864819867362No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT110430.6811994983687114No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA106670.6580055283074385No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT106050.654180990690952No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT100940.6226593984002329No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT93110.5743591894694441No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA93090.5742358172882671No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA87450.5394448621963579No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA84470.5210624072009875No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA81410.5021864634809091No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT70080.4322961228441482No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA68860.4247704197923523No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT66000.4071281978840437No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT65980.4070048257028667No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGATCATTGT64700.39910900610753985No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA63880.3940507466792835No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAAT55010.3393351843272916No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT53170.3279849436590092No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA52650.32477726694840764No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT47560.29337904683886545No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGATCATTGT44080.2719122873140704No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT43490.26827280796934944No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA43380.26759426097287603No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGTGACGGCGACCAGGGC41150.2538382627716424No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT40780.25155587741986823No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT36730.22657301073152916No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAAT34270.2113982324467603No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA33660.20763538092086228No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT33220.20492119293496866No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGT33000.20356409894202185No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT32970.20337904067025636No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT32960.20331735457966787No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC31690.1954832210749295No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT30940.19085676428079262No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGTGACGGCGACCAGGGC29030.17907472097839075No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA26110.16106238252655125No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT25950.16007540507713539No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT25780.159026741537131No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA25130.15501714564887908No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT25030.15440028474299416No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT24530.15131598021356957No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA23540.14520905724530894No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT23100.1424948692594153No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA23040.14212475271588434No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACAGTGACCGTGGT22410.13823852900880937No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCATCGT22000.13570939929468123No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGT21500.13262509476525666No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA21240.13102125640995588No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT20860.12867718496759323No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA19540.12053462100991233No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT19390.11960932965108496No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGGGGT19100.11782043302401872No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA19070.11763537475225323No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC19050.11751200257107625No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGT18730.11553804767224453No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA17710.1092460664322184No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCGTCGT17210.10616176190279382No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT16560.10215216601454188No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)