FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266509
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT899197.099752153360142No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT860776.7963986043525955No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT593224.683898811615235No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT529494.180704598230253No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA511734.040476617221038No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT423533.344074143965815No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA376792.9750282074584544No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA376322.9713172192222874No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT285792.256517719179256No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA184011.4528913730577515No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA159361.2582618836502544No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA142121.1221396768597776No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCATCGT140971.113059599260645No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA129721.0242327531821724No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA123220.9729105754479439No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCATCGT105020.8292084777921042No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT104750.8270766334862208No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA91740.7243533208212496No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT76810.6064702264255525No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT73080.5770191921257567No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT70670.557990507765835No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT70540.5569640642111505No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA67490.5328821192743202No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGATCATTGT65940.5206437538146196No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT57420.4533722223845231No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT53820.42494763163941196No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT50710.4003919435234965No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA49800.3932068386407045No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAATGT49240.38878523563590944No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT48370.38191595953917423No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGATCATTGT44630.3523859680428643No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT43690.3449639915705297No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT43260.3415688321204192No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA42830.3381736726703087No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA42550.3359628711679112No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA42530.33580495677488276No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGT41970.3313833537700877No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGT41840.33035691021540314No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT41770.32980420983980374No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGGGGT41200.3253036496384945No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT38020.30019526114697964No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC36970.29190475551298883No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT36390.28732523811516536No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAAT35850.28306154950339873No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT34180.26987569768552766No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA33890.267585938986616No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTAAGGAGACGGTGACCAGGGT32550.2570056746537135No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAATGT32500.2566108886711425No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGAT32180.25408425838268817No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA31360.24760976826852396No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCGTCGT31220.2465043675173252No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA30420.24018779179618938No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGTGT28940.22850212671208808No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA28110.22194867940140972No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT28020.22123806463278192No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT28000.22108015023975353No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT27880.22013266388158315No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA27820.21965892070249798No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT27450.21673750443147263No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT27290.2154741892872455No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGT27070.21373713096393315No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGACACCGTCACCATCGT26520.20939448515565226No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGGGGT25800.20370956700663No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT25720.20307790943451645No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT25050.1977877772680652No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGTGGT24800.1958138473552103No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA24370.19241868790509975No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGAT24090.19020788640270223No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC23980.18933935724104606No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA23970.18926040004453187No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA23740.1874443845247053No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCGTCGT23550.18594419779093554No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCAGGGT23480.18539149741533617No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAAT22890.1807330228209985No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACCGCGACCATCGT22530.1778905637464874No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTAAGGAGACGGTGACCAGGGT21740.17165294522186578No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT20780.1640730543565028No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGACACCGTCACCATCGT19810.15641420629462563No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT19220.15175573170028794No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA18970.14978180178743303No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT18420.14543915597915213No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT18070.1426756541011552No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA18030.14235982531509844No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT17930.14157025334995646No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACCGCGACCATCGT17580.13880675147195953No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGT17190.1357274208079058No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACAGTGACCGTGGT17140.13533263482533484No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCAGGGT16390.12941084508677No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT16040.1266473432087731No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGTGGT15430.12183095422140704No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT15090.11914640953992431No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA15050.11883058075386752No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT15030.11867266636083912No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGTGACGGCGACCAGGGC14370.11346149139090209No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT13650.10777657324187984No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT13210.10430245659525515No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA12870.10161791191377242No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)