FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135836
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT823157.2470849664916415No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA647665.702055578446184No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA637705.614366862821745No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA627705.526325983680742No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA557664.90968766617716No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT541734.769438545705542No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA285082.5098693825517064No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA256912.2618582260115017No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA215221.8948158008726612No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA190771.6795558513729096No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA165421.4563722227504674No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT153441.3508992495395462No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGCT115371.0157276226497487No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT105850.9319127057075142No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT105460.928479111421015No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA93280.8212453206272736No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGATACGGTGACCGTGGT88170.7762564313862212No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA84330.7424487337960762No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGCT77730.6843417535630144No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT76910.6771224014734522No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA76420.6728083983955431No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT71230.6271151821213625No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT71140.6263228142090935No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT67030.5901380128821414No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGATACGGTGACCGTGGT56930.5012167249497286No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA55980.4928528414313334No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT54370.478678259889632No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA54070.4760370335154019No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT52920.46591233241418656No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA52610.4631830651608155No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT46870.41264760053387994No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA45560.4011142453664085No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA44230.38940480844065517No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA43510.38306586514250296No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT42100.3706521011836216No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA41080.3616719315112393No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA39930.35154723041002395No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT39700.3495222901897809No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGTTGT37540.33050546029532435No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCATGGT37270.32812835655851724No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT36220.31888406424871196No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA35550.3129853253462648No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGT33980.2991629073211273No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCGGGGT33260.29282396402297517No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA32110.2826992629217598No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT31490.2772407284150177No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGACGT31070.27354301149109556No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA30770.27090178511686547No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA30460.2681725178634944No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA28100.24739487038621774No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT28050.24695466599051272No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT27000.23771037368070744No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC26560.2338365749985033No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT26270.2312833895034142No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGTTGT23860.21006553763043256No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA23840.20988945587215058No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCATGGT23600.2077764747727665No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCGGGGT22900.20161361323289628No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGACGT22080.19439426114333408No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA21670.19078458509855298No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGAT21370.18814335872432286No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGT20890.18391739652555475No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA20340.1790751481727996No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT19050.16771787476361025No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT18860.16604509805993117No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA18780.16534077102680317No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA18450.16243542201515007No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT18240.160586563553189No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT17950.15803337805809994No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT17640.15530411080472886No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA17310.15239876179307577No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC16890.14870104486915364No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA16370.1441229191538215No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA15680.13804809849309232No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT15170.13355801365690118No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA14700.12942009233727403No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT14650.12897988794156903No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT14170.1247539257428009No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTAGT14020.12343331255568585No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGT13690.12052796354403277No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA13630.11999971826918675No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT13600.11973559563176375No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGAT13570.11947147299434073No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA13220.11639004222440563No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTG13070.11506942903729059No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA12700.1118119165090735No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA12620.11110758947594547No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT12240.10776203606858736No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA12170.10714574991460034No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC12060.10617730024404931No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT12050.10608925936490832No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT11860.10441648266122927No Hit
GNNAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA11720.10318391035325522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)