FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398393
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT905036.4719288497582586No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA779565.57468465588715No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT582684.166782871481765No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA538353.8497761358931286No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA514043.675933732505812No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA454063.2470128211454146No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT405302.8983268651945484No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA347692.486353979174667No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT300782.1508974944811654No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT270461.9340771871712743No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT204801.4645382235179953No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA185071.3234477003245868No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA175501.2550120030635163No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA169041.2088161196459077No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA146371.0467014637516063No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA144311.0319702687298922No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT107770.7706703337330779No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT76560.5474855780885631No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT73090.5226713806490736No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT69990.500503077461057No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA68590.49049158569872703No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGATCAGGGT68300.4884177766908159No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA62410.44629800063358444No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT61680.4410777227860837No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT60410.4319958695445415No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA58700.41976754746340983No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC57960.4144757589604639No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT53800.3847273262952546No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA52510.37550245174282193No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT51230.3663490878458345No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA50840.3635601722834711No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT49220.35197544610134635No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT48950.3500446584043255No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGT46270.33087980274500806No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA45520.32551650358661693No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGATCAGGGT45500.3253734822757265No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT44910.3211543536044588No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT43780.31307364953914957No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA41340.2956250496105172No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT40850.2921210274937017No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT40050.28640017505808457No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT38870.27796191771554923No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC38320.2740288316660624No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA37700.2695951710284591No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA37230.26623417022253404No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA36810.263230722693835No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGT36540.2612999349968142No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT35390.2530762096206145No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA35020.25043031536914157No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT34660.24785593177311388No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT32330.23119394905437884No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA31430.22475799006430952No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA31090.22232662777917225No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGTGACGATGACCGTGGT30620.21896562697324715No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT30470.21789296714156892No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT29760.21281571060495869No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA29270.2093116884881432No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGT29120.20823902865646496No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT29090.20802449669012932No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA28210.2017315590109504No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT28100.20094494180105307No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT26970.19286423773574382No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCATGGT26710.19100496069416822No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGATGACCGTGGT26710.19100496069416822No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT26650.19057589676149694No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA26340.1883590664426953No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA25490.18228066072985205No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT25060.1792057025457078No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT24440.17477204190810453No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGGCCATGGT23860.17062442389228208No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGT23660.1691942107833778No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGTGACGATGACCGTGGT23460.16776399767447348No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT22330.15968329360916425No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA22060.15775250591214343No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA21530.15396244117354707No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT21380.15288978134186884No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGT20360.14559569448645696No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC20060.14345037482310052No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT19870.14209167236964143No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT19770.1413765658151893No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT19300.1380155650092642No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT19290.13794405435381898No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT18400.13157960601919488No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT17850.12764651996970808No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT17680.12643083882713943No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT17570.1256442216172421No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCATGGT17340.12399947654200213No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT17330.12392796588655694No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT16950.12121056097963877No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA16510.11806409214004931No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGATACGGTGACCGTGGT15680.11212870773809652No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGGCCATGGT15470.11062698397374701No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA15190.108624685621281No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGT14660.10483462088268461No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA14620.10454857826090377No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT14560.10411951432823248No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT14540.10397649301734206No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCT14430.10318987580744468No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTAGT14270.10204570532032124No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)