FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939350
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT710117.559589077553627No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT438644.669611965720978No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA328653.4986959067440253No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT153581.6349603449193593No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT147941.5749188268483525No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA142161.5133869164848035No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT112761.200404535050833No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT96891.0314579230318839No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT94591.0069729067972533No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA81370.8662372917442913No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA73190.7791557992228669No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT68130.7252887635066801No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT67890.7227338052908926No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT66140.7041039016341087No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA60210.6409751423856923No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA59390.6322457018150849No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT59100.629158460637675No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA58350.621174216213339No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT52330.5570873476340023No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA48240.51354660137329No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT45680.4862937137382233No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT42150.4487145366476819No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT41100.4375365944536115No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT37410.39825411188587856No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT35700.38005003459839254No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT35530.3782402725288763No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC30990.3299089796135626No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT30700.32682173843615264No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACCATGACCAGGGT29220.3110661627721297No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA28840.30702081226379946No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT28640.30489168041730985No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGTTGT27320.29083941023047855No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT26860.28594240698355244No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT25060.2667802203651461No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA25000.2661414808111992No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGT24570.2615638473412466No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA24240.2580507797945388No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT24150.2570926704636185No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT23880.2542183424708575No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA22550.24005961569170173No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA22480.23931441954543037No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT21590.22983978282855166No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT20960.22313301751210943No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT20560.21887475381913024No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA20250.21557459945707136No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA19700.209719486879225No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC19510.20769681162505987No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACCATGACCAGGGT18430.19619949965401606No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCGTTGT17230.1834247085750785No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACAATGACCATTGT16910.18001809762069515No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT16880.1796987278437217No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA16510.17575983392771596No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCAGGGT16240.17288550593495502No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT16120.17160802682706125No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT15860.16884015542662478No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA15450.16447543514132112No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT15290.16277212966412946No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT15190.16170756374088466No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC15070.1604300846329909No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGT15020.1598978016713685No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT14580.1552137116090914No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT14150.15063607813913876No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA13990.1489327726619471No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT13790.1468036408154575No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT13260.14116144142226006No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCATTGT12900.1373290040985788No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGAT12810.13637089476765848No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT12430.13232554425932824No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT12200.12987704263586522No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT12140.12923830308191833No Hit
GCCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT11800.12561877894288603No Hit
TACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT11700.12455421301964123No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA11480.12221216798850268No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGATGAGACGATGACCGTGGT11120.11837973066482141No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACAATGACCATTGT11020.11731516474157663No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGTT10990.11699579496460319No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCAGGGT10720.11412146697184224No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGT10460.11135359557140576No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGT10390.1106083994251344No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT10310.10975674668653856No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT10190.1084792675786448No Hit
TACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT10180.10837281098632033No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCAGGGT10010.10656304891680417No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGT9830.10464683025496353No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT9790.10422100388566562No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGT9730.10358226433171873No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT9660.1028370681854474No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT9560.1017725022622026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)