FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314534
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT18002313.694815044723075No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1160258.826321723135347No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA613484.666900970229754No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAACCTGAAGAGACGATGAC202371.5394809111061412No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA197531.5026617797637793No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT173061.3165121632456824No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA169071.2861592016638597No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA165001.2551976593986918No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA163421.2431781909026318No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCT161141.2258336414273043No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT154331.1740282107575764No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAACCTGAAGAGACGATGAC151681.1538689756217793No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACAGT132411.0072771035210957No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT123730.9412460993781827No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA122160.9293027034675405No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT116030.8826702086062437No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCT112170.8533061906348562No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA111790.8504154323889682No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGT108040.8218882128571797No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT106280.8084994378235937No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT100220.7623994510602237No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA97480.7415555626556636No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGT91300.6945427048672762No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT91080.6928691079880779No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT86030.654452452351936No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA85670.6517138392768844No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACAGT83660.6364232496078458No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA76230.5799013186421956No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT76160.5793688105442689No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT75110.5713811890753682No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGT67490.513413878986774No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGT67420.5128813708888473No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGT64840.4932546438509769No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA63130.4802462317444813No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA55090.4190838730683269No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT54690.41604096965160275No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA50430.38363404826349107No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGC49070.37328817664662917No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGTGGT46620.354650393219194No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGATCAGGGT45240.3441523764314959No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTCACCGTGGT42540.3236127783686082No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA42070.3200373668539574No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT40900.3111368743600394No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCCGAGACGGTGACCAGGGT39180.29805238966812575No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA36010.2739373800905872No Hit
GNNAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT34770.26450437949874256No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGC33810.2572014112986047No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGATGACCGTGGT32870.25005058826930304No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGTGGT31380.23871577304200578No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGATCAGGGT30320.23065207898768691No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA29760.22639201420427313No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACGAGGGT28110.21384003761028622No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTCACCGTGGT27840.21178607780399744No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTAAAGAGACGGTGACCATTGT27750.21110142453523453No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCCGAGACGGTGACCAGGGT26990.20531990804345873No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT26470.20136413360171743No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT25450.19360472988907096No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGATGACCGTGGT24810.1887360844223124No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT24050.1829545679305366No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT23480.17861843056170476No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGCTGACCAGGGT23060.17542338197414445No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGGCCAGGGT22720.17283691406992896No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT22310.16971793806778676No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGT22050.1677400508469161No Hit
GNNAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT21930.16682717982189885No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTCACCAGGGA20980.15960028420717912No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACGAGGGT20060.15260160634871367No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTAAAGAGACGGTGACCATTGT19990.15206909825078696No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT18520.1408864281943259No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT18460.1404299926818173No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGT18270.13898461355887334No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC17930.13639814565465785No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT17430.13259451638375272No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAACCTGAAGAGACGATGAC17290.13152950018789927No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGT17090.13000804847953723No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA16760.12749765316073985No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT15940.12125970115645544No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGCTGACCAGGGT15240.11593462017718828No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT15170.11540211207926156No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT15030.1143370958834081No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT15000.11410887812715381No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGGCCAGGGT14640.11137026505210212No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA14570.1108377569541754No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTCACCAGGGA14440.10984881334374005No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA14320.10893594231872283No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA13970.10627340182908926No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT13850.10536053080407201No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGTCT13770.10475195012072719No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACCGTGACCAGGGT13760.10467587753530908No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAACCTGAAGAGACGATGAC13740.10452373236447289No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAACCTGAAGAGACGATGAC13700.10421944202280047No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT13230.10064403050814964No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA13220.10056795792273156No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)