FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672409
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1215607.268556914008475No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1120676.700932606796544No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT719344.301220574632162No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT546823.2696547315877873No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA418122.5001061343247972No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT361082.1590412393140674No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA315471.8863208700742464No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT279951.6739326325079569No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA270531.6176066978831134No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA207701.241921085093419No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA200361.1980322995152501No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT184211.1014650124461183No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT158730.9491099366243545No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA157410.9412171304985802No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA137710.8234229784699796No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT111350.6658060318976996No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA109760.6562987881552897No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT103970.6216780703763254No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT102240.611333710832697No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT97440.5826325976480634No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA82500.49330038286089106No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA79180.47344877957485276No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT78800.47117660811440265No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT78000.466393089250297No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA75830.4534177943314106No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT73940.442116731014961No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGT69140.4134156178303274No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT68820.4115022102846851No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGT67020.4007392928404475No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT63430.37927325193777356No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGT63160.37765881432113796No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT62800.3755062308322904No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA60190.3599000005381459No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA58560.35015358085253073No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGAT57500.3438154183575908No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGGT55190.3300030076374858No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT54490.3258174286313934No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT50710.3032153019984944No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA49320.2949039379721109No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT48440.28964206722159475No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCAGGGT48230.288386393519767No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT46430.27762347607552934No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGT46330.27702553621751613No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT42870.2563368171302594No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT42300.2529285599395842No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCAGGGT42280.2528089719679815No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC42260.25268938399637886No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAAGGACCAGGGT40680.2432419342397703No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGAT37880.22649961821540068No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT36870.22046042564946733No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGAGGT36710.21950372187664619No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGGT36660.21920475194763958No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT35890.21460061504093797No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA35720.2135841172823155No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT35130.2100562721200376No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGTGACGGTGACCAGGGT31540.1885902312173637No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT31280.18703558758652936No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT30700.1835675364100528No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT30110.1800396912477749No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAAGGACCAGGGT27690.16556954668385543No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT27250.16293861130859735No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT26870.16066643984814719No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA26310.15731797664327327No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC25990.15540456909763103No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA25460.15223548785016106No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGTT25420.15199631190695576No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT25300.15127878407733994No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGAGGT24410.14595711934102246No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT24040.1437447418663736No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23640.1413529824343208No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC23630.14129318844851949No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT23480.14039627866149965No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGT23420.14003751474669174No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGT23250.13902101698806932No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGAT22880.13680863951342045No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA22810.13639008161281121No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA22590.13507461392518216No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA22360.1336993522517518No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGT21540.12879624541604356No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGT21250.12706221982780527No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGATGGTGACCATTGT21060.1259261340975802No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGT20940.12520860626796435No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT20440.12221890697789835No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGATCAGGGA20370.12180034907728911No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCTGGGT20310.12144158516248119No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA20250.12108282124767328No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT19770.11821270992920989No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA19730.11797353398600463No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA19730.11797353398600463No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT19510.11665806629837558No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGACGGCGACCATTGTCCC19180.11468486476693202No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT18600.11121681359045545No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT18320.10954258198801847No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGT18060.10798793835718415No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGT17720.10595494283993927No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGAGCATTGT17700.10583535486833663No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT17690.1057755608825353No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA17420.10416112326589967No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT17000.10164977586224423No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA16830.10063327810362178No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT16750.10015492621721121No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)