FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768750
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1285357.266996466431095No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1027305.808056537102473No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT828104.681837455830388No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA735574.158699646643109No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA639993.618318021201414No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA372492.1059505300353356No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT339861.921469964664311No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA280071.5834346289752652No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT266111.5045088339222614No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA264191.4936537102473497No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA241891.3675759717314488No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT225541.2751378091872791No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT195541.1055265017667844No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA181591.0266572438162545No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT176380.9972014134275617No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA164130.9279434628975266No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT131160.7415406360424028No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA112360.6352508833922261No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT112170.6341766784452297No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA104450.590530035335689No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC101580.574303886925795No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA90630.5123957597173145No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA89450.5057243816254416No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT85770.48491872791519436No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGTCGACGGTGACCGTGGT78690.4448904593639576No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT74100.4189399293286219No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT70140.3965512367491166No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA69970.3955901060070671No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC63220.35742756183745583No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT62670.3543180212014134No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA61730.34900353356890457No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGTCGACGGTGACCGTGGT54430.30773144876325087No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT53920.3048480565371025No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA53450.3021908127208481No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA52970.29947703180212015No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT51060.28867844522968195No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA49710.2810459363957597No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA47070.2661201413427562No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGCGT41060.23214134275618376No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT40780.2305583038869258No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA40410.22846643109540637No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT36620.20703886925795054No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA35750.2021201413427562No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT32990.18651590106007065No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT32740.18510247349823322No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCTGGGT32250.1823321554770318No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA31720.1793356890459364No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT31700.1792226148409894No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGAGT31590.17860070671378092No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA31110.175886925795053No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA29850.16876325088339222No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGT29710.16797173144876326No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT29300.16565371024734982No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGCGT26030.1471660777385159No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT25120.14202120141342756No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGT23900.13512367491166077No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA23860.13489752650176678No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC23790.1345017667844523No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA23520.13297526501766785No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT23210.1312226148409894No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA23130.1307703180212014No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGT23050.13031802120141342No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT22050.1246643109540636No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT22020.12449469964664311No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT22010.12443816254416962No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTG21640.12234628975265018No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGGCGT21370.12081978798586573No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA21060.11906713780918729No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT20840.11782332155477032No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCTGGGT20820.11771024734982333No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA20400.1153356890459364No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGT20090.11358303886925794No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT19780.11183038869257951No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT18880.10674204946996466No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGT18390.10397173144876325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)