FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906714
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23664912.41135272515962No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC995015.218454367041938No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC941844.939597653344969No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC701113.6770590660161933No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC606043.178452562890921No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC535232.807080663382133No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC505992.6537278270364615No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC421172.208878730632911No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC345131.8100774421334294No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC312411.6384733106276033No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC234391.2292876645369992No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC210621.104622927193066No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC178460.935955785713012No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC142700.7484079940672801No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC129490.679126497209335No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAAATTCC120240.6306137155336354No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC117940.6185510779277857No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT116740.6122575278725598No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC101300.5312805171619865No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC99830.5235709183443348No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC74530.3908819046799887No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGTTCC64270.33707205170780724No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTTCC60520.3174047077852263No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC59550.3123174214905854No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC57680.302509972654525No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC56750.29763247136172494No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC51120.2681052323526234No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAAATTCC50320.26390953231580616No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG40100.21030946434546557No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC38960.20433059179300095No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC32780.17191880900858755No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC32580.17086988399938324No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG30670.160852650161482No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGTTCC27180.14254890875086668No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC25140.13184987365698264No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC24610.1290702223825912No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGGGGTGCC24060.12618567860727933No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC22480.11789917103456522No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTTCC22390.11742715478042329No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21740.11401814850050926No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21640.1134936859959071No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGGCGT20440.1072001359406812No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA20220.10604631843055645No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20100.10541696342503387No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC19910.10442048466628975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)