FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524092
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC33850422.210207782732276No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17284711.34098204045425No Hit
AAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCACGGTTCC679714.459770145109351No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC385282.5279313847195577No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC190931.252745897229301No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC187861.2326027562640576No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC182301.19612201888075No Hit
AAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCACGGTTCC159401.0458686221041775No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC103740.6806675712489797No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT85870.5634174314936369No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC70340.46152069560105297No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCT65770.4315356290827588No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC62210.40817745910351866No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC61620.4043063017193188No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC54520.35772118743487924No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC52390.3437456531495474No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC42740.28042926542492186No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC38100.2499849090474853No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCT34180.22426467693551308No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC32990.21645674933009293No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC32650.21422591287140147No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC31380.20589308256981861No Hit
AAGACGGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC27880.18292858961270053No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC26210.1719712458303042No Hit
AAGACCGATGGCCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC25880.16980602220863308No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC25600.16796886277206363No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC25450.16698467021675856No Hit
AAGACCGATGGACCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC24720.16219493311427394No Hit
AAGACGGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21780.14290475903029476No Hit
AAGATCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20170.13234109227002044No Hit
AAGACGGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC15930.1045212493733974No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC15840.10393073384021437No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAGCACTGTGCC15380.10091254333727884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)