FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342633
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC946087.0464527536564345No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC713825.316568265490272No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC487603.6316700096005383No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC424613.1625172329296243No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC314322.341071610782693No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC270072.011495322995934No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC251661.8743766911732394No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCGTGGTCCC231361.7231812416349068No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC189021.4078307325978134No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTAAAGAGACGATGACCAGGGTTCC176441.3141342421942557No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC158341.1793245063989937No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC133070.9911122399047244No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTAGTCCC129790.9666826303241467No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC122140.9097050348084698No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC116760.8696345166549608No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC116600.8684428283827376No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC94670.70510705457113No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTAAAGAGACGATGACCAGGGTTCC92010.6852952370454175No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC83440.6214654339644564No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCGTGGTCCC79740.5939076426692924No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC77770.5792349808175428No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGCATTCC70860.5277689435608985No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTAGTCCC69820.5200229697914471No Hit
AAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCACGGTTCC69080.5145114115324143No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC67400.5019986846740696No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC64890.48330407490356636No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCGTGGTCCC51060.38029751987326393No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGGGGTTCC50860.3788079095329848No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC43010.32034070367702866No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCGTGGTCCC42270.31482914541799584No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC41360.3080514183697257No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG36850.2744607051964312No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGCATTCC36630.2728221338221241No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC35250.2625438224741981No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC33780.25159518647314644No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGGGGCTCC33660.25070142026897896No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC28210.21010953849637243No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGGGGTTCC25160.18739298080711556No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCTGGGAACC24120.17964700703766406No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTTCCTCTGGGAGCT23180.1726458384383521No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTGCC21960.16355921536264936No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC21630.16110135830118877No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAATTCC20070.1494823976470115No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCGGAGACGGTGACCGTGGTCCC19120.1424067485306856No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGGGGCTCC18610.1386082421629738No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC17270.12862785288310358No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG16120.12006259342649854No Hit
AAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCACGGTTCC15830.11790265843309378No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC15370.11447655465045177No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC13580.10114454210495348No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)