FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482982
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC857485.7821335660176585No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC655294.418731987306657No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC450723.0392816635670563No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC446683.0120392560395204No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC419892.831389726915094No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC387172.610753198622775No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC342882.312098191346894No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC327092.205623534203382No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC271071.8278711407151267No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC216711.4613124097258092No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC192631.298936871789408No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC148260.9997424108991207No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC145740.9827496220453114No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC144760.97614131526883No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC106700.7194962582148671No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC98770.6660229186868081No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC90550.610594059806525No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT86710.5847002863150059No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC84120.5672354755485906No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC83320.5618409394045242No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC74210.500410659063967No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC67140.45273644589077955No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATTCGGGTTCC62730.4229990653966131No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC61490.41463753437331No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGGGTTCC57290.38631621961696094No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGACGAGACGGTGACCAGGATTCC55200.3722229939405873No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGTCCC49730.3353378530555327No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC49220.3318988362636903No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC49160.33149424605288536No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC41600.28051587949145707No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC35260.2377641805497302No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC34460.2323696444056637No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC32390.2184112821328917No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC30590.2062735758087421No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGACGAGACGGTGACCAGGATTCC27690.18671838228650112No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCAGTGTCCC26070.1757944465947665No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC25690.17323204192633493No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC25610.17269258831192827No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGGGTTCC25170.1697255934326917No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC24530.1654099645174385No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC24260.1635893085688161No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATTCGGGTTCC23320.15725072859953795No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC23090.15569979945811885No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGTCCC22660.15280023628068312No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC21520.1451130222753884No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC20050.1352005621106662No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC19380.13068263809001054No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC18380.12393946790992742No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC18050.12171422175049999No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTCGTCCC18010.12144449494329668No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC15680.10573290842370306No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC15140.10209159652645819No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACTCGGGTTCC14940.10074296249044155No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)