FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702968
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC644413.784040569171No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC639633.755971926659808No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC592093.4768122477932644No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC557173.2717584828370234No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC517873.0409849157470954No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC442622.59910931972885No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC340251.9979823461157227No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC328801.9307467903096243No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC319201.8743746212494892No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC267371.5700236293341978No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC249571.4655002325351973No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC246141.4453589262980866No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC232721.3665553316327728No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC214051.2569232070126979No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC209101.2278563073410658No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC181261.0643770170666742No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTTCC138760.8148127269567015No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC110680.6499241324558066No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC104220.6119903603590907No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGACGAGACGGTGACCAGGATTCC101640.5968403399241795No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC83450.49002682375711115No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC83220.4886762405400454No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC81010.4756988974543268No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC68300.4010644944590856No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC67150.39431157837375685No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTTCC67080.39390053130769337No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAATGTCCC67010.39348948424162994No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC65110.3823324924484782No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGGGTTCC62430.3665952619191905No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC52500.30828529954761336No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC50030.2937812102165161No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC49870.29284167406551387No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC47420.27845502675329187No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGACGAGACGGTGACCAGGATTCC45660.2681201290922672No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC45370.2664172198185756No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT42560.24991661616659855No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGGCGGTGACCAGGGTTCC39380.23124333516542883No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC35930.2109845869094428No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC35040.20575841706949277No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC32450.19054967562514386No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG30910.18150664017174722No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCAGTGTCCC30840.18109559310568374No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCAGGGTTCC30200.17733744850167474No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTCCC28880.16958627525590617No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC28800.16911650718040502No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTCGTCCC28370.16659150377458648No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAATGTCCC28200.1655932466141466No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGGGTTCC26930.15813567841556625No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC26440.15525834895312185No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC26370.15484730188705836No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC25430.14932752699992013No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATTCGGGTTCC24100.14151763274471393No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTTGTCCC23800.1397560024615847No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC21610.12689610139474142No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGAGTTCC21510.126308891300365No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC21250.12478214505498636No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAACGTTCC20490.12031934833772566No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC20370.11961469622447397No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC19730.11585655162046499No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC19360.11368387427127227No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGTTCC19340.113566432252397No Hit
AAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC18930.11115887086545373No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCCTGTCCAAGCTGC18710.10986700865782562No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACAAGGGTTCC18440.10828154140300934No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC18190.10681351616706831No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC18110.10634374809156719No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC17820.10464083881787563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)