FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718489
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC1079636.282437653077791No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC906445.2746337043763445No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC574563.3434022562844454No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC428352.492596693956144No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC427922.490094495804163No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC380152.212117738315462No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC310331.8058305872193536No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC309841.8029792451391893No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC307901.7916902581279253No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC258461.5039956613047858No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC243441.416593297949536No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC233811.3605556974760968No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC211711.231954350595203No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC177911.0352699377185424No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC141170.8214774723608937No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC119030.6926433628612112No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC116640.6787357963885716No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC110340.6420756839293124No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC97390.5667187860963905No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGGGTTCC93160.5421041391594592No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC92140.5361686923803411No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC91150.5304078175653146No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC84760.49322398921378025No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC80220.46680543198123464No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCAGTGTCCC78560.4571457833014933No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC77240.4494646168814581No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT71370.41530670257418No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC64590.3758534386894533No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTCCC55750.3244128999370959No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC51480.29956549038137575No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC49460.28781097813253387No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTCGTCCC45930.26726967702440924No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGACGAGACGGTGACCAGGATTCC43640.253944017098742No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC40010.23282080944364494No Hit
AAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC38320.22298658879981192No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACTGTGACCAGGGTTCC38010.2211826785041976No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCAGTGTCCC36330.2114066485150618No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC35810.20838073447080546No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATTCGGGTTCC34100.19843013251757796No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA32960.19179639788209293No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG31460.1830677996775074No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC30360.17666682766081132No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCCTGTCCAAGCTGC28600.16642527243409763No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG27750.16147906678483248No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTCCC26480.1540888536382834No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC26280.15292504054433864No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC25290.14716416572931218No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC24920.14501111150551443No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC23910.1391338553810935No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTTCC22390.1302888758671135No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC20910.12167665897192242No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACAGTGACCAGGGTTCC19840.11545025891931808No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC19640.11428644582537333No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC19300.11230796356566729No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGACGAGACGGTGACCAGGATTCC19230.11190062898278662No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC19150.11143510374520872No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTGCC18830.10957300279489715No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTCGTCCC18380.10695442333352148No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC17700.10299745881410936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)