FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780086
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC992835.577427158013714No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC954225.360527525074631No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGCGGTCCC572053.2136087806993596No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC546763.0715369931565104No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC438962.465948274409214No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC437922.4601058600539525No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC405482.2778674738186804No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC326291.8330013269021834No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC312381.7548590349005608No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC300301.6869971450817545No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC263681.48127674730322No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGCGGTCCC243951.3704394057365767No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC239961.3480247583543716No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC204261.1474726501977994No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC186861.049724563869386No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC154090.865632334617541No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC148350.8333867015413862No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC136600.7673786547391531No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC120300.6758100451326509No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC111600.6269360019684442No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC103590.5819381760207091No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC102090.5735116168544666No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC80250.45082091539397534No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGCTCC73940.4153731898346485No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC73350.41205874322925967No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC66230.3720606757201618No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGATGACCAGGGTTCC64800.3640273559816773No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGCGGTCCC64410.3618364505984542No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTCCC53070.2981316633016607No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC51540.28953657295209334No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGATGGTGACCGTGGTCCC49500.2780764524860035No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC44160.24807790185418008No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC38160.21437166518920997No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGTTCC37760.2121245827448786No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTTCC36590.20555186659520944No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC36060.2025744823564704No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC35840.2013385870120882No Hit
AAGACCGATGGGCCCTTGGTGGAGGAGTAATAGACAGCCGTGTCTTCAGT33500.18819315471274983No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGCTCC32830.18442929161849483No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC30360.17055355752474882No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGAGGAGACGGTGACCAGGGTTCC30350.17049738046364052No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC29510.1657785073305447No Hit
AAGACGGATGGGCCCTTGGTGGAGGGTGAGGAGACGATGACCAGGGTTCC28530.16027315534193293No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC27810.15622840694213652No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACAAGGGTTCC27210.1528577832756395No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGCGGTCCC27100.15223983560344836No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGTCCC24910.1399370592207343No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC24910.1399370592207343No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGGGACCAGGGTTCC24260.13628555024869585No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGGGGAGATGGTGACCGTGGTCCC23630.13274639539887398No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGACGTCCC23320.13100490650451718No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC23310.1309487294434089No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACACTGACCGACGTCCC23310.1309487294434089No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC21570.12117392081056758No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC19760.11100587274996826No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC18840.10583758312800617No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTATTGTCCC18360.10314108419480857No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18010.10117488705601865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)