FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633171
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1467138.983321403576234No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC674924.132573992558036No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC453232.775153367283646No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC329022.0146083906706647No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC304421.8639811752719098No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC295281.808016429387982No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC276101.6905761858372454No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGAAGACCGATGTCCC234831.437877601304456No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC212771.3028029520484996No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC200101.2252238130606041No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACGAGGGTTCC181901.1137841658956715No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC163010.9981196090305302No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC159820.9785870554889843No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC143280.8773116838346995No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC141440.8660452579674756No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC132440.8109377401386628No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGCGGTCCC131030.8023042290121487No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGAAGACCGATGTCCC104870.6421250438563996No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGACCACTGTCCC99200.6074073076242475No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC96600.5914873580292572No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATTCGGGTTCC91140.5580554638797774No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC86390.5289709405812373No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC85970.5263992564158927No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACGAGGGTTCC85390.5228478830447026No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGTTCC74960.4589843929386451No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTGCC68660.4204091304584762No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC68310.418266060320689No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC65770.40271349417789076No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC62570.38311971006097956No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGCGGTCCC58530.3583825576133791No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC46890.28711016788811455No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC45150.27645604777454413No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATTCGGGTTCC43180.26439362442757064No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCAGTGTCCC42650.2611484039332072No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACAATGACCATTGTCCC42080.25765826113738244No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC40000.24492230146139016No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC36100.2210423770689046No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTTCC34740.2127150188192173No Hit
AAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGTTCC34090.20873503142046973No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTGCC32770.20065259547224384No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC32590.1995504451156676No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC32110.19661137749813093No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC31760.1944683073603438No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC30990.18975355305721203No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC29920.1832018814931198No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC29390.1799566609987564No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGTTCC28790.17628282647683555No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGTAGTCCC27720.16973115491274335No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26980.16520009233570765No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC26920.16483270888351556No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGCTCC26360.1614037966630561No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCAGACCGATGTCCC24320.1489127592885252No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23610.14456538843758554No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCGTGGTCCC23270.1424835488751637No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC23240.14229985714906768No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC23070.14125893736785675No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT22250.13623803018789826No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGTTCC21030.12876789999332586No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGATGATGACCGTGGTCCC20640.12637990755407733No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC20320.1244205291423862No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCAGTGTCCC19570.11982823598998511No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACAATGACCATTGTCCC19560.11976700541461978No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC19410.11884854678413956No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC18860.11548086513904546No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC18490.1132153338505276No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18190.11137841658956715No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC17320.10605135653278194No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC16540.10127537165428482No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCGTGGTCCC16470.1008467576267274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)