FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679555
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC360985.3120056507567455No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC336414.950445512136619No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC333774.911596559513211No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC253883.735974277284399No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC209593.084224234977301No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC201842.9701790142078273No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC174802.5722715600650425No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC157422.3165159552942733No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC147012.1633274716542443No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC107451.5811818027974192No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC92841.3661881672565135No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC86481.272597508663758No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC85561.2590592372949945No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC83111.2230062320194834No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC76401.1242651441016547No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT50830.7479894931241768No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC46440.6833883938754037No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC43370.6382117709383347No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC39890.5870017879347514No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC37530.5522731787714018No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC36960.5438853367277116No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC35320.5197518964616551No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC32220.47413380815386535No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC31110.4577995894372053No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA26730.3933456453120056No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC26690.3927570248177116No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC26590.39128547358197646No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC24580.3617072937436999No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC17740.2610531892194156No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC17360.2554612945236221No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC17060.25104664081641664No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC13890.2043984666436124No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC13880.20425131152003884No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG13410.19733502071208364No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC13090.19262605675773117No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG12000.17658614828821803No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC10820.15922184370654324No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACACTGACCAGGGTTCC10050.1478908991913826No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGTGGTCCC8390.1234631486781791No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCGTGGTCCC7980.11742978861166498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)