FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451052
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC677934.671989701265013No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC627044.3212786309518885No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC591994.079729740905219No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC561003.8661605511036132No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC440363.0347637438217236No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC432352.979562414027891No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC380662.6233381022871685No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC284791.9626450327073048No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC269201.8552057403869744No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC249661.7205448185178753No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC191581.3202834908742072No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC158251.0905880698968748No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC130390.8985894371807489No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT116350.8018320501263911No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC96880.6676535368822069No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC88820.6121076294991497No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC73870.5090789303208982No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC67750.4669026333997679No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC62900.4334786072449506No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC61400.4231412795682029No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGAGAGACGCTGACCGTGGTCCC55250.38075823609353765No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA42520.2930287818768728No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC41740.287653371484964No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC40940.28214013005736527No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC38850.2677367868277636No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC38800.2673922092385387No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC37060.25540090913351143No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC34310.23644914172614076No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC33250.22914409683457243No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC31680.2183243605329099No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC30690.2115017242662565No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC30660.21129497771272152No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGATGACCGCTGTCCC30420.20964100528444188No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG29130.20075090348243896No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC26640.18359093953903788No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGGAGAGACGCTGACCGTGGTCCC24350.16780928595253652No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG22670.15623147895457914No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCGTGGTCCC21790.15016691338422056No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC18690.12880310285227545No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCCGTACT18640.12845852526305052No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC18410.1268734683526159No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC17930.12356552349605666No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTCCAGAGACGGTGACCATTGTCCC17640.12156697347855212No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC16300.11233229408732423No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC16290.11226337856947925No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGTGGTCCC15580.10737037680248536No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC15090.10399351642808115No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC15070.10385568539239118No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC14710.10137472674997175No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)