FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381927
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC815355.900094578078292No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC739235.349269534497843No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC578434.185676956887013No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC407912.9517478130176196No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC394642.855722480275731No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC353742.559758945298847No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC353422.557443338179224No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC239751.7348962716554492No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC190391.3777138734535181No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC155531.1254574228595289No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC126220.9133622832465101No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC124720.9025078748732749No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGCGTTCC118630.8584389768779392No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTCCC108750.7869446070595625No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTTCC88590.6410613585232794No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGTTTCC76650.5546602678723261No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC75000.5427204186617672No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC72970.5280307859966554No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC72860.5272347960492848No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC72670.5258599043220082No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC71460.5171040149009318No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC67880.49119816025014346No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC55020.3981396991302725No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC53090.38417369369004295No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTCCC51100.36977351191488406No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT47470.34350584365165454No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC43620.31564619549368383No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTTCC42250.30573250251279555No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC41610.30110128827354843No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGTCCC38720.28018846147444837No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGTTCC37480.2712154838859071No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGTTTCC36840.2665842696466601No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC35570.25739420389065415No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC35290.25536804766098353No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC35050.2536313423212659No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC29170.21108206149818334No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCATTGTCCC28920.2092729934359774No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATGGTCCC28600.20695738631635394No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGGTGACCAGGGTTCC27840.201457819407248No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACGAGGGTTCC27820.20131309396227154No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC26500.19176121459382442No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC26320.19045868558903617No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC25260.1827882370052832No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC24510.17736103281866553No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGCGTTCC22470.16259903743106546No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC22200.1606452439238831No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCGTGGTCCC21110.1527577071726654No Hit
AAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCAGGACTCC21070.1524682562827125No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCGGAGACGGTGACCGTGGTCCC20610.14913957104825362No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC20470.14812649293341834No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGCTGACCAGGGTTCC20440.14790940476595363No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAATGACCGGGGTTCC20220.14631742487121244No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC19900.1440018177515889No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC19320.13980477984727124No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTGGTCCC18630.13481175199558298No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGTTCC18540.13416048749318885No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC16080.1163592577610829No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGTCCC16030.11599744414864172No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTTGATGGCACAGAAGT15590.11281348435915936No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC14870.10760336834000639No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCATTGTCCC14310.10355105588066518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)