FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757170
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC1005895.7244888087094585No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC751964.279381050211419No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC709354.036888861066374No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC676323.848916154953704No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC641543.6509842530887737No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC625783.5612945816284136No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC559003.181251671722144No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC454692.587626695197391No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC382772.1783322046244815No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC380962.168031550732143No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC301561.7161686120295703No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC300701.7112743786884592No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC292611.6652344394680083No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC284281.617828667687247No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC222871.2683462613179146No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC160490.9133436150173291No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACTAGGGTGCC143510.8167109613753933No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC119210.6784204146440014No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC116530.6631686177205393No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT104630.5954460866051663No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC90350.5141790492667186No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC68850.3918232157389439No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC62410.3551733753706244No Hit
AAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACTAGGGTGCC57200.32552342687389385No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC56370.3207999226028216No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGTCCC55630.31658860554186563No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC53340.3035562865289073No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC50420.2869386570451351No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC49800.28341025626433414No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC43640.24835388721637636No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGTTTCC34030.19366367511396165No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC34020.19360676542394872No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA28060.1596885901762493No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGAGGTCCC27770.1580382091658747No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTGTGGTCCC23010.13094919671972546No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC22800.12975409322945416No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC20590.1171770517365992No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC19050.10841295947460974No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC18420.10482764900379586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)