FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609084
Sequences flagged as poor quality0
Sequence length250
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20810912.933383216786693No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1054676.5544744711898195No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC922985.736058527708932No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC522503.2471890839757283No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC457232.8415545739066452No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC412552.5638810652520316No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC268621.6693969985407848No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA209581.302480168841403No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC194481.2086379579934918No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA176941.0996318402271106No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC176441.0965244822520142No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA115140.7155623945051968No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC97210.6041325375182401No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGAGGTGGGGGTCTCAGCCCTTCCT77100.47915459975986335No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC74230.4613183649828101No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA73120.4544200302780961No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG71790.44615445806433973No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC71230.44267421713223176No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC67740.42098485846605893No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC64950.4036458009650211No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC53980.33547036699140625No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTATGTGCCCCTCCTGGATGAGCTC48720.30278096109339225No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC43890.2727638830539611No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGACGCTGACCACACGGTACG40930.2543683238413905No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC40160.24958299255974206No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGAGGTGGGGGTCTCAGCCCTTCCT39790.24728354765817073No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCACTTG39030.24256036353602425No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCACCACAGGCTCTGGCTGACTC38760.24088239022947217No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC37540.23330043677023699No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC37070.2303795202736464No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC36290.225532041832496No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC33590.2087523087669755No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC28000.17401204660539785No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT26040.16183120334302No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTTGGTCCTGGTACCC26010.16164476186451424No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTATGTGCCCCTCCTGGATGAGCTC23040.14318705549244165No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21610.13430001168366598No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGACGCTGACCACACGGTACG21390.13293277417462357No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC21070.1309440650705619No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20960.13026044631604067No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19790.12298922865431512No Hit
AAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTCTGG18660.11596659963059729No Hit
AAGACGGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18410.1144129206430491No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18230.11329427177201438No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC18040.11211347574147776No Hit
AAGACGGATGGGCCCTTGGTGAGGAGACAGATGGGGCATGCCTTGCTGCC17860.11099482687044306No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTCATTTTTGTTTTTGCAGCACTTG17270.10732814445982933No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCGGCGGCTCTGCCCTGGTCCCC16800.10440722796323872No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)