FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459625
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1049037.186982957951529No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC637954.370643144643315No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC547233.7491136422026208No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC420772.8827267277554167No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC338622.3199109360280894No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC219361.5028517598698297No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTGCC215711.47784533698724No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC183951.2602552025348976No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC182971.2535411492677915No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC116480.7980131883189177No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTGCC112410.7701293140361394No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTGCC109620.7510148154491736No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC105280.7212811509805601No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGCTCC87860.6019354286203648No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC81340.557266421169821No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGCGACCAGAGTTCC80900.5542519482743855No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTTCC78790.53979618052582No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC78720.5393166052924553No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC77070.5280123319345722No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC71380.4890297165367817No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCTGGGTTCC68280.46779138477348636No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGACGAGACGGTGACCAGGGTTCC65420.44819731095315574No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC60820.4166823670463304No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC58600.401472981073906No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTGCC57890.3966087179926351No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC56110.38441380491564614No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC55120.3776312409009163No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC55000.37680911192943395No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGTGGCTGCTCGGTCCTGGTACCC54070.3704376124004453No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGTGTTCC53340.3654363278239274No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC50190.34385544232251436No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC47200.3233707287830778No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGCTCC45550.3120664554251948No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT45450.3113813479489595No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC43530.29822728440524104No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGCGACCAGAGTTCC43220.2961034512289115No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC42050.2880876937569581No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTTCC41780.2862379035711227No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC41360.2833604521709343No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTCACCAGGGTTCC41200.2822642802089578No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC37950.2599982872313094No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCTGGGTTCC36410.24944763209728527No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC36390.24931061060203819No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC34630.2372527190202963No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC31100.21306842510918902No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC30980.2122462961377066No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC29920.20498415688961205No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC29590.20272330221803547No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCACTGTCCC29380.20128457651794127No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCACTGTCCC28840.19758499614627045No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGCTGACCGTGGTCCC28040.19210413633638776No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGTGTTCC27820.19059689988867004No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC26370.1806628414832577No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA25770.17655219662584568No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTTATCAAGGTTCC22970.15736918729125632No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGATTCC22590.15476577888156204No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTCACCAGGGTTCC22460.1538751391624561No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCTGAGTTCC21420.14675002140960863No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19730.13517170506123147No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC18960.1298963774942194No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18910.12955382375610175No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCAGGGTTCC18510.1268133938511604No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC17560.12030487282692472No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCACTGTCCC15820.1083840027404299No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGCTGACCGTGGTCCC15210.10420484713539435No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCGTGGTCCC15190.1040678256401473No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACTTGGGTTCC14710.10077930975421769No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC14640.10029973452085296No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC14600.10002569153035883No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)