FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038864
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1437277.049366706165787No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC1009024.94893234664009No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC820294.02326982084141No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC762943.741985733231839No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC746263.6601754702618714No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC668143.277020929301807No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC555562.724850701174772No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC400481.9642310620031547No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC340411.6696062120867308No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC294521.4445298950788281No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC264941.2994491049917993No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC240011.17717513281906No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGACACTGTAACCAGGGTTCC151270.7419327625579735No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC141140.6922482323489944No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC124940.6127922215508244No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC114360.560900579930785No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT110460.5417722810349292No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC105630.5180826185562156No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGGAGAGACGGTGACCAGGGTTCC88820.4356347456230528No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC81550.3999776346043679No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG81070.39762338243257034No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC78130.38320358788030984No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAGGACACTGTAACCAGGGTTCC76760.3764841598066374No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC75230.36897998100903245No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC56950.27932221079973946No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC55730.27333848652975384No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC54920.26936568598984534No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC49820.24435175666449555No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTTACCGGGGTTCC49040.2405260968853244No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGGAGAGACGGTGACCAGGGTTCC44110.2163459652041529No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGTGGTCCC43300.21237316466424438No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC39130.1919205989217525No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG37610.18446546704439334No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC35200.17264515926515941No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGGAGACGGTGACCAGGGTTCC35020.1717623147007353No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC32290.1583725054736363No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC30650.15032881055332775No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGACCC30310.14866121526497109No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC28970.14208892795203604No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC27360.13419237379246482No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26350.12923863484764064No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC25470.12492250586601165No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC25200.1235982390193755No Hit
AAGACCGATGGGCCCTTGTTGGAGGCTGAGGAGACGGTGACCAGGGTTCC25140.1233039574979008No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGTGTTCC24940.12232301909298512No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTCCC23940.11741832706840673No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC23780.11663357634447417No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTTACCGGGGTTCC23080.11320029192726931No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC23000.11280791656530303No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC22550.11060080515424275No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC21900.1074127553382668No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC21840.10711847381679208No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGTTCC21780.1068241922953174No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCGTGGTCCC21360.10476422164499447No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC21280.1043718462830282No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC21200.10397947092106191No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)