FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001880498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998479
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1291976.4647664548889425No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC747593.740794874502059No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC684623.4257052488417443No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC637533.190076052838183No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC598202.9932763866920795No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC488552.444609125239745No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC468672.3451334740069822No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC432542.164345985121685No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC426692.1350737235667725No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC298461.4934357578938784No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC296821.4852295170477148No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC248971.2457974289447125No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCCGGGTTCC223021.115948678970357No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC216891.085275351905124No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC185040.9259041501061558No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCCGGGTTCC117290.5868963346625109No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC114420.5725354131817247No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC109090.5458651304316933No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT102160.5111887590512585No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC98300.49187407023041024No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGCTTCC80820.40440755194325284No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGGCCAGGGTTCC77040.38549316755392476No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC75390.3772368886538212No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC71420.35737178123963276No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC67410.33730652160968416No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC64970.32509723644831895No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC56730.28386588000174134No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTTCC50690.2536428954219684No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCTGGGTTCC50520.25279224850498805No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACAGTGACCGTTGTCCC50500.252692172397108No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGCTTCC41730.20880879909170924No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC41300.20665716277228832No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC38760.1939474970715229No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGGCCAGGGTTCC38460.19244635545332225No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGCCTTCC37270.1864918270344597No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC37090.18559114206353933No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC36520.1827389729889581No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGAGACGGTGACCAGGGTTCC33850.16937881258697238No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC33120.16572603464935085No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGTTCC32900.16462519746267035No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG32410.1621733328196093No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTTCC32170.1609724195250488No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG31820.15922108763714804No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGGGGTCCC29370.1469617644218428No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTTCC28720.14370929091574142No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCTTTCC28600.14310883426846116No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACAGTGACCGTTGTCCC27740.13880556162961932No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCTGGGTTCC27140.13580327839321804No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC25270.12644616230643405No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGATGAGGAGACGGTGACCAG23220.11618836124872967No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGTTTCC22990.11503748600810916No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC22430.11223535498746796No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA22370.11193512666382785No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGCCGGTGACCAGGACTCC22140.11078425142320736No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC21670.10843246288802634No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGCTGACCAGGGTTCC21560.10788204429468612No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC21240.10628082656860542No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC20880.10447945662676467No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC20670.1034286574940242No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGATTCC20530.1027281247388639No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)