FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786193
Sequences flagged as poor quality0
Sequence length250
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC1473648.250172293811476No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC947065.302114609115588No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC678323.7975739463764557No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC655673.6707679405305025No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC618243.4612161171833056No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC580373.2492009542081957No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC562243.1477001645398897No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC554903.1066071807469853No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC331601.8564623195813665No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC311891.7461159012491931No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC282211.5799524463481829No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC266421.4915521447010485No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC142930.8001934841307743No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC140230.7850775364140382No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT115030.6439953577245012No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC84610.47368901344927455No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC81130.454206236392148No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC73780.41305726760769973No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC67180.3761071731890115No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC62700.3510258969775382No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC51360.28753891656724667No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC50860.2847396669900733No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC46390.25971437577014356No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGTTCC36890.20652863380384986No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAGTGGTCCC31300.1752330235310518No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC29410.16465186012933652No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC29310.16409201021390185No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC29250.16375610026464105No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG25310.14169801359651504No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC24960.1397385388924937No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC24380.1364914093829726No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC24240.13570761950136406No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC22480.12585426098971386No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTGCC20490.11471324767256394No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC18540.10379617432158786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)