FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709324
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1018925.960952984922694No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC575293.3655995001532766No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC453732.654441170895629No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC346172.025186564981244No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC302761.7712265199575972No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC286631.6768617301342519No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC250141.4633855255059895No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC209341.224694674619908No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC203521.1906461267729231No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC182321.0666204885674102No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC181251.0603607039976037No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAATTCC160480.9388506801519197No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC155960.9124074780439518No Hit
AAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCCGGGTGCC155920.9121734674058283No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCTGGGTCCC149560.8749657759441745No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC122960.7193487015919744No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC110770.6480339596238045No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGAGGACTGTGGTCCC81870.47896127357949697No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC78170.4573152895530631No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC75520.44181208477737394No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAGAGGAGACGACGACCAGGGTCCC74030.4330951885072695No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAATTCC72850.4261918746826231No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCATTGTCCC72410.4236177576632634No Hit
AAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCCGGGTGCC69540.4068274943778944No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC68890.4030248215083858No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCTGGGTCCC64930.37985776833414847No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACTGTGGTCCC63590.3720184119570076No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGACTTCC62960.3683327444065607No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC60430.35353157154524245No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC59740.3494948880376102No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACCATGACCAGGGTCCC52270.3057934013680262No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC51190.2994751141386887No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC49160.28759907425391557No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC47690.278999183302873No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGTTCC46040.2693462444802741No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGATTCC45700.2673571540562234No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTTCC44050.2577042152336245No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGTCTCC39180.22921342004207512No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGAGGACTGTGGTCCC35970.2104340663326555No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC35150.2056368482511215No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGATTCC35020.20487631367721976No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACACTGACCAGGGTGCC34480.20171717006255108No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC34170.19990358761709306No Hit
AAGACCGATGGGCCCTTGGTGGAGGCAAAAGAGACGGTGACCAGCGTTCC33640.19680294666195525No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC33460.19574989879039903No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGACTCC33430.19557439081180633No Hit
AAGACGGATGGGCCCTTGGTGGAGGCAGAGGAGACGACGACCAGGGTCCC32660.19106968602792684No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG32530.1903091514540251No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAAGAGACGGTGACCATTGTCCC30990.18129974188626616No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACTGTGGTCCC28350.16585503977010796No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGATCAGGGTTCC27810.16269589615543922No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC27300.15971226051936321No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGAGAGACGATGACCGTTGTCCC27270.15953675254077052No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGACTTCC26970.15778167275484345No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC26520.15514905307595284No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAACGACCAGACTTCC26240.15351097860908758No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGGGACCAGGGTTCC25610.14982531105864072No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC25220.14754370733693553No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC24880.14555461691288485No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCGTGGTCCC24350.14245397595774703No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCGTTGTCCC23180.1356091647926315No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC23070.13496563553779156No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC22730.13297654511374088No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACCATGACCAGGGTCCC22450.1313384706468756No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC22040.12893986160610862No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGATTCC22030.1288813589465777No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC21930.1282963323512687No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGCTGGATCCTCAG21800.12753579777736695No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGATTCC21110.1234991142697347No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCGCAGTTCC20690.12104200256943681No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGCGACGGTGACCAGGGTTCC19780.1157182605521247No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGCGCC19330.11308564087323408No Hit
AAGACGGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGTTCC19050.11144756640636884No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGGACGCTGAC18910.11062852917293621No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACCGTGACCATTGTCCC18880.11045302119434348No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG18510.10828842279170012No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18280.10694286162248937No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGCCCC17420.10191163290283177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)